Product nameAnti-NOSIP antibody
See all NOSIP primary antibodies
DescriptionRabbit polyclonal to NOSIP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide within Human NOSIP aa 1-50. The exact sequence is proprietary.
Database link: Q9Y314
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241401 was affinity purified using an epitope specific to NOSIP immobilized on solid support.
Our Abpromise guarantee covers the use of ab241401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
RelevanceNOSIP is a novel type of modulator that promotes translocation of eNOS from the plasma membrane to intracellular sites, thereby uncoupling eNOS from plasma membrane caveolae and inhibiting NO synthesis.
Cellular localizationCytoplasm. Nucleus. Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle.
- CGI 25 antibody
- CGI25 antibody
- CGI25 protein antibody
All lanes : Anti-NOSIP antibody (ab241401) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
ab241401 has not yet been referenced specifically in any publications.