Overview

  • Product name

  • Description

    Rabbit polyclonal to NOSIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human NOSIP aa 1-50. The exact sequence is proprietary.
    Database link: Q9Y314

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab241401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.

Target

  • Relevance

    NOSIP is a novel type of modulator that promotes translocation of eNOS from the plasma membrane to intracellular sites, thereby uncoupling eNOS from plasma membrane caveolae and inhibiting NO synthesis.
  • Cellular localization

    Cytoplasm. Nucleus. Note: Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle.
  • Database links

  • Alternative names

    • CGI 25 antibody
    • CGI25 antibody
    • CGI25 protein antibody
    • eNOS interacting protein antibody
    • Nitric oxide synthase interacting protein antibody
    see all

Images

  • All lanes : Anti-NOSIP antibody (ab241401) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

References

ab241401 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241401.
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