Overview

  • Product name

    Anti-Notch1 antibody [mN1A]
    See all Notch1 primary antibodies
  • Description

    Mouse monoclonal [mN1A] to Notch1
  • Host species

    Mouse
  • Specificity

    ab128076 recognizes intracellular domain of Notch1 protein, mainly its activated form. The unprocessed Notch1 protein is recognized with lower affinity.
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Fusion protein corresponding to Mouse Notch1. GST fusion protein containing cdc10-NCR region of mouse Notch1
    Database link: 4851

Properties

Applications

Our Abpromise guarantee covers the use of ab128076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 272 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • Tissue specificity

    In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • Involvement in disease

    Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • Sequence similarities

    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization

    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9930111A19Rik antibody
    • AOS5 antibody
    • AOVD1 antibody
    • hN1 antibody
    • Lin-12 antibody
    • LIN12 antibody
    • Mis6 antibody
    • Motch A antibody
    • mT14 antibody
    • Neurogenic locus notch homolog protein 1 antibody
    • Neurogenic locus notch protein homolog antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • NOTCH antibody
    • Notch gene homolog 1 (Drosophila) antibody
    • Notch homolog 1, translocation-associated (Drosophila) antibody
    • NOTCH, Drosophila, homolog of, 1 antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation associated notch homolog antibody
    • Translocation associated notch protein TAN 1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    • xotch antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab128076 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128076, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Lin Y  et al. Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells. Oncol Rep 39:687-694 (2018). Read more (PubMed: 29251326) »
  • Wang P  et al. Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway. Stem Cell Res Ther 8:114 (2017). IF, IHC ; Rabbit . Read more (PubMed: 28511663) »
See all 9 Publications for this product

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