For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    notch1-antibody-n25332-ab167441.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Notch Pathway
Share by email

Anti-Notch1 antibody [N253/32] (ab167441)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [N253/32] (ab167441)
  • Western blot - Anti-Notch1 antibody [N253/32] (ab167441)

Key features and details

  • Mouse monoclonal [N253/32] to Notch1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Notch1 protein (ab114178)
Primary
Product image
HRP Anti-Notch1 antibody [EP1238Y] (ab194123)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Notch1 antibody [N253/32]
    See all Notch1 primary antibodies
  • Description

    Mouse monoclonal [N253/32] to Notch1
  • Host species

    Mouse
  • Specificity

    ab167441 detects >270kDa, ~120kDa and additional small fragments due to proteolysis. ab167441 does not cross-react with Notch2 or Notch3.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Fusion protein corresponding to amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of Mouse Notch1 (UniProt Q01705).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain membrane lysate.
  • General notes

    The clone number has been updated from S253-32 to N253/32, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N253/32
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Embryogenesis
    • Somatogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant Human Notch1 protein (ab114178)

Applications

Our Abpromise guarantee covers the use of ab167441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 271 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • Tissue specificity

    In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • Involvement in disease

    Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • Sequence similarities

    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • Cellular localization

    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Target information above from: UniProt accession P46531 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 18128 Mouse
    • Entrez Gene: 25496 Rat
    • SwissProt: Q01705 Mouse
    • SwissProt: Q07008 Rat
    • Unigene: 290610 Mouse
    • Unigene: 25046 Rat
    • Alternative names

      • 9930111A19Rik antibody
      • AOS5 antibody
      • AOVD1 antibody
      • hN1 antibody
      • Lin-12 antibody
      • LIN12 antibody
      • Mis6 antibody
      • Motch A antibody
      • mT14 antibody
      • Neurogenic locus notch homolog protein 1 antibody
      • Neurogenic locus notch protein homolog antibody
      • NICD antibody
      • NOTC1_HUMAN antibody
      • Notch 1 antibody
      • Notch 1 intracellular domain antibody
      • NOTCH antibody
      • Notch gene homolog 1 (Drosophila) antibody
      • Notch homolog 1, translocation-associated (Drosophila) antibody
      • NOTCH, Drosophila, homolog of, 1 antibody
      • notch1 antibody
      • TAN1 antibody
      • Translocation associated notch homolog antibody
      • Translocation associated notch protein TAN 1 antibody
      • Translocation-associated notch protein TAN-1 antibody
      • xotch antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [N253/32] (ab167441)
      Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [N253/32] (ab167441)

      Immunocytochemistry/Immunofluorescence analysis using ab167441 at 1/100 in Neuroblastoma cell line (SK-N-BE). Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubation for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain 1/1000 for 60 min at RT; DAPI (blue) nuclear stain at 1/5000 for 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab167441 (D) Composite.

       

    • Western blot - Anti-Notch1 antibody [N253/32] (ab167441)
      Western blot - Anti-Notch1 antibody [N253/32] (ab167441)
      Anti-Notch1 antibody [N253/32] (ab167441) at 1/1000 dilution + Rat Brain membrane lysate. at 10 µg

      Secondary
      HRP conjugated Goat Anti-Mouse IgG at 1/200 dilution

      Predicted band size: 271 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab167441? Please let us know so that we can cite the reference in this datasheet.

    ab167441 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab167441.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.