Product nameAnti-Notch2 antibody
See all Notch2 primary antibodies
DescriptionRabbit polyclonal to Notch2
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Notch2 aa 2396-2409 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Synthetic peptide:
NAAERTPSHSGHLQconjugated to KLH with a Cysteine residue linker, corresponding to amino acids 2396-2409 of Human Notch2 (NP_077719.2).
(Peptide available as
- Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride, 98% Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab118824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400 - 1/2000. Predicted molecular weight: 265 kDa.|
|ELISA||1/30000 - 1/90000.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Tissue specificityExpressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
Involvement in diseaseAlagille syndrome 2
Sequence similaritiesBelongs to the NOTCH family.
Contains 6 ANK repeats.
Contains 35 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
Cellular localizationCell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
- Information by UniProt
- AGS2 antibody
- hN2 antibody
- Motch B antibody
This product has been referenced in:
- Xue F et al. Neferine inhibits growth and migration of gastrointestinal stromal tumor cell line GIST-T1 by up-regulation of miR-449a. Biomed Pharmacother 109:1951-1959 (2019). Read more (PubMed: 30551450) »
- López-Arribillaga E et al. Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells. Nat Commun 9:2992 (2018). Read more (PubMed: 30065304) »