Anti-Notch2 antibody (ab137665)

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Western blot - Anti-Notch2 antibody (ab137665)
  • Western blot - Anti-Notch2 antibody (ab137665)

Key features and details

  • Rabbit polyclonal to Notch2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Notch2 antibody
    See all Notch2 primary antibodies

  • Description

    Rabbit polyclonal to Notch2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Notch2 aa 1669-1855.

  • Positive control

    • A431 and HepG2 cell lines; NIH-3T3 and HeLa whole cell lysate (ab150035).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rat
Application Abreviews Notes
WB
1/500 - 1/300. Predicted molecular weight: 265 kDa.
Notes
WB
1/500 - 1/300. Predicted molecular weight: 265 kDa.

Target

  • Function

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).

  • Tissue specificity

    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.

  • Involvement in disease

    Alagille syndrome 2
    Hajdu-Cheney syndrome

  • Sequence similarities

    Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.

  • Post-translational
    modifications

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.

  • Cellular localization

    Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt

  • Database links

    • Alternative names

      • AGS2 antibody
      • hN2 antibody
      • Motch B antibody
      • N2 antibody
      • N2ECD antibody
      • N2ICD antibody
      • neurogenic locus notch homolog protein 2 antibody
      • NOTC2_HUMAN antibody
      • Notch 2 antibody
      • Notch 2 intracellular domain antibody
      • Notch Drosophila homolog 2 antibody
      • Notch homolog 2 antibody
      • Notch homolog 2 Drosophila antibody
      • Notch2 antibody
      see all

    Images

    • Western blot - Anti-Notch2 antibody (ab137665)
      Western blot - Anti-Notch2 antibody (ab137665)
      Anti-Notch2 antibody (ab137665) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 265 kDa



      5% SDS PAGE
    • Western blot - Anti-Notch2 antibody (ab137665)
      Western blot - Anti-Notch2 antibody (ab137665)
      Anti-Notch2 antibody (ab137665) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

      Predicted band size: 265 kDa



      5% SDS PAGE

    References (6)

    Publishing research using ab137665? Please let us know so that we can cite the reference in this datasheet.

    ab137665 has been referenced in 6 publications.

    • Cao M  et al. Emodin relieves hypoxia-triggered injury via elevation of microRNA-25 in PC-12 cells. Artif Cells Nanomed Biotechnol 47:2678-2687 (2019). PubMed: 31257935
    • Li F  et al. LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through Up-Regulating KLF2 Expression in ATDC5 Chondrocytes. Cell Physiol Biochem 45:1241-1251 (2018). PubMed: 29448248
    • Liang Z & Ren C Emodin attenuates apoptosis and inflammation induced by LPS through up-regulating lncRNA TUG1 in murine chondrogenic ATDC5 cells. Biomed Pharmacother 103:897-902 (2018). WB ; Mouse . PubMed: 29710506
    • Yang Z  et al. Down-regulation of microRNA-23b aggravates LPS-induced inflammatory injury in chondrogenic ATDC5 cells by targeting PDCD4. Iran J Basic Med Sci 21:529-535 (2018). PubMed: 29922435
    • Zhou J  et al. Protective role of microRNA-29a in denatured dermis and skin fibroblast cells after thermal injury. Biol Open N/A:N/A (2016). WB ; Human . PubMed: 26794609
    • Liu YF  et al. Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells. Mol Med Rep 13:41-8 (2016). PubMed: 26549737

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