Overview

  • Product name
  • Description
    Rabbit polyclonal to Notch2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Notch2 aa 1669-1855. Synthetic peptide, corresponding to a region within amino acids 1669-1855 of Human Notch2

  • Positive control
    • A431 and HepG2 cell lines; NIH-3T3 and HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab137665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/300. Predicted molecular weight: 265 kDa.

Target

  • Function
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • Tissue specificity
    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • Involvement in disease
    Alagille syndrome 2
    Hajdu-Cheney syndrome
  • Sequence similarities
    Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • Cellular localization
    Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS2 antibody
    • hN2 antibody
    • Motch B antibody
    • N2 antibody
    • N2ECD antibody
    • N2ICD antibody
    • neurogenic locus notch homolog protein 2 antibody
    • NOTC2_HUMAN antibody
    • Notch 2 antibody
    • Notch 2 intracellular domain antibody
    • Notch Drosophila homolog 2 antibody
    • Notch homolog 2 antibody
    • Notch homolog 2 Drosophila antibody
    • Notch2 antibody
    see all

Images

  • Anti-Notch2 antibody (ab137665) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 265 kDa



    5% SDS PAGE
  • Anti-Notch2 antibody (ab137665) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 265 kDa



    5% SDS PAGE

References

This product has been referenced in:
  • Liang Z & Ren C Emodin attenuates apoptosis and inflammation induced by LPS through up-regulating lncRNA TUG1 in murine chondrogenic ATDC5 cells. Biomed Pharmacother 103:897-902 (2018). WB ; Mouse . Read more (PubMed: 29710506) »
  • Zhou J  et al. Protective role of microRNA-29a in denatured dermis and skin fibroblast cells after thermal injury. Biol Open N/A:N/A (2016). WB ; Human . Read more (PubMed: 26794609) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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