Overview

  • Product name

  • Description

    Rabbit polyclonal to Notch2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Notch2 aa 2421-2471. The exact sequence is proprietary. NP_077719.2
    Database link: Q04721

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • Tissue specificity

    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • Involvement in disease

    Alagille syndrome 2
    Hajdu-Cheney syndrome
  • Sequence similarities

    Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • Cellular localization

    Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt
  • Database links

  • Alternative names

    • AGS2 antibody
    • hN2 antibody
    • Motch B antibody
    • N2 antibody
    • N2ECD antibody
    • N2ICD antibody
    • neurogenic locus notch homolog protein 2 antibody
    • NOTC2_HUMAN antibody
    • Notch 2 antibody
    • Notch 2 intracellular domain antibody
    • Notch Drosophila homolog 2 antibody
    • Notch homolog 2 antibody
    • Notch homolog 2 Drosophila antibody
    • Notch2 antibody
    see all

Images

  • Notch2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245325 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab245325 at 1 µg/ml.

    Lane 1: ab245325 IP in HeLa whole cell lysate.

    Lane 2: Control IgG in HeLa wholoe cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-Notch2 antibody (ab245325) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Exposure time: 3 minutes
  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Notch2 using ab245325 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab245325 has not yet been referenced specifically in any publications.

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