Key features and details
- Rabbit polyclonal to Notch2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Notch2 antibody
See all Notch2 primary antibodies
DescriptionRabbit polyclonal to Notch2
The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.
Tested Applications & Species
Application Species IHC-PHuman
Synthetic peptide corresponding to Human Notch2 aa 1719-1733 (N terminal) (acetyl C) (Cysteine residue). The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
(Peptide available as
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Tissue specificityExpressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
Involvement in diseaseAlagille syndrome 2
Sequence similaritiesBelongs to the NOTCH family.
Contains 6 ANK repeats.
Contains 35 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
Cellular localizationCell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
- Information by UniProt
- AGS2 antibody
- hN2 antibody
- Motch B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
ab8926 has been referenced in 47 publications.
- Zhao J et al. MicroRNA-204-5p inhibits the osteogenic differentiation of ankylosing spondylitis fibroblasts by regulating the Notch2 signaling pathway. Mol Med Rep 22:2537-2544 (2020). PubMed: 32705191
- Jiang H et al. Long non-coding RNA SNHG3 promotes breast cancer cell proliferation and metastasis by binding to microRNA-154-3p and activating the notch signaling pathway. BMC Cancer 20:838 (2020). PubMed: 32883233
- Song Y et al. The ethyl acetate extracts of radix trichosanthis protect retinal vascular endothelial cells from high glucose-induced injury. J Ethnopharmacol 240:111954 (2019). PubMed: 31085225
- Yu Y et al. MiR-490-5p inhibits the stemness of hepatocellular carcinoma cells by targeting ECT2. J Cell Biochem 120:967-976 (2019). PubMed: 30206962
- Yang J et al. Role of Jagged1/STAT3 signalling in platinum-resistant ovarian cancer. J Cell Mol Med 23:4005-4018 (2019). PubMed: 30993885