Overview

  • Product name
  • Description
    Rabbit Polyclonal to Notch2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Notch2 aa 1719-1733 (N terminal) (acetyl C) (Cysteine residue). The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
    Sequence:

    CRDASNHKRREPVGQD


    (Peptide available as ab1389, ab205702)

Properties

Applications

Our Abpromise guarantee covers the use of ab8926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/30000 - 1/90000.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20706108
WB Use at an assay dependent concentration.

Target

  • Function
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • Tissue specificity
    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • Involvement in disease
    Alagille syndrome 2
    Hajdu-Cheney syndrome
  • Sequence similarities
    Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    modifications
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • Cellular localization
    Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS2 antibody
    • hN2 antibody
    • Motch B antibody
    • N2 antibody
    • N2ECD antibody
    • N2ICD antibody
    • neurogenic locus notch homolog protein 2 antibody
    • NOTC2_HUMAN antibody
    • Notch 2 antibody
    • Notch 2 intracellular domain antibody
    • Notch Drosophila homolog 2 antibody
    • Notch homolog 2 antibody
    • Notch homolog 2 Drosophila antibody
    • Notch2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.

  • Staining of Human corneal epithelial cells with anti-Notch2 (ab8926, 1/100). The Notch2 (green staining) is localised in the cytoplasm. The nucleus is stained with Bis benzimide (1/500)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

References

This product has been referenced in:
  • Wu XZ  et al. Knockdown of lncRNA PVT1 inhibits retinoblastoma progression by sponging miR-488-3p. Biomed Pharmacother 112:108627 (2019). Read more (PubMed: 30797143) »
  • Zhang J  et al. miR-29a/b cluster suppresses high glucose-induced endothelial-mesenchymal transition in human retinal microvascular endothelial cells by targeting Notch2. Exp Ther Med 17:3108-3116 (2019). Read more (PubMed: 30936982) »
See all 33 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver progenitor)
Permeabilization
Yes - Triton X-100
Specification
Liver progenitor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 21 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (postnatal day 1 testes)
Specification
postnatal day 1 testes
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Qing Wen

Verified customer

Submitted Dec 19 2012

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