Key features and details
- Rat monoclonal [NIZ 8G5] to NOTCH3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-NOTCH3 antibody [NIZ 8G5]
See all NOTCH3 primary antibodies
DescriptionRat monoclonal [NIZ 8G5] to NOTCH3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant full length protein corresponding to Human NOTCH3 aa 1-2321.
Database link: Q9UM47
- T47D or Jurkat cell extract
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Clone numberNIZ 8G5
Our Abpromise guarantee covers the use of ab213632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 244 kDa.|
FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Tissue specificityUbiquitously expressed in fetal and adult tissues.
Involvement in diseaseDefects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) [MIM:125310]. CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.
Sequence similaritiesBelongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 34 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
- Information by UniProt
- CADASIL antibody
- CASIL antibody
- NOTC3_HUMAN antibody
ab213632 has not yet been referenced specifically in any publications.