This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-NOTCH4 antibody (ab121004)


  • Product name
  • Description
    Goat polyclonal to NOTCH4

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
  • Species reactivity

    Predicted to work with: Mouse, Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:


    N, corresponding to internal sequence amino acids 1261-1273 of Human NOTCH4 (NP_004548.3). The immunizing peptide represents part of the extracellular domain.



Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. Preliminary experiments gave an approx 27kDa band in all Human, Mouse and Rat tissue and cell line lysates tested after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide.


  • Function
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
  • Tissue specificity
    Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 28 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • Post-translational
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
  • Cellular localization
    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16302 antibody
    • hNotch 4 antibody
    • hNotch4 antibody
    • INT3 antibody
    • Neurogenic locus notch homolog protein 4 antibody
    • NOTC4_HUMAN antibody
    • Notch 4 antibody
    • Notch 4 intracellular domain antibody
    • Notch homolog 4 antibody
    • NOTCH3 antibody
    • Notch4 antibody
    • NOTCH4 protein antibody
    see all


ab121004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HEK293, NIH3T3, MCF7 and MDA-MB-231)
Loading amount
20 µg
HEK293, NIH3T3, MCF7 and MDA-MB-231
Gel Running Conditions
Reduced Denaturing (7-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Anthea Messent

Verified customer

Submitted May 31 2012

Thank you for your inquiry and your call.

I can now confirm that this antibody was tested on the cell lines HEK293 and NIH3T3 plus small intestines and heart lysates from human, mouse and rat. The 27kDa was blocked by the immunizing peptide. ...

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