Recombinant Anti-NOTCH4 antibody [EPNCIR101B] (ab166605)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR101B] to NOTCH4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-NOTCH4 antibody [EPNCIR101B]
See all NOTCH4 primary antibodies -
Description
Rabbit monoclonal [EPNCIR101B] to NOTCH4 -
Host species
Rabbit -
Specificity
Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested. -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Mouse NOTCH4 aa 1900-2000. The exact sequence is proprietary.
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Positive control
- Mouse lung lysate and Jurkat cells
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General notes
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPNCIR101B -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/100 - 1/500. Predicted molecular weight: 210 kDa.
Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested. |
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/100 - 1/500. Predicted molecular weight: 210 kDa. Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested. |
Target
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Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. -
Tissue specificity
Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. -
Sequence similarities
Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 28 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
Post-translational
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Phosphorylated. -
Cellular localization
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4855 Human
- Entrez Gene: 18132 Mouse
- Omim: 164951 Human
- SwissProt: Q99466 Human
- SwissProt: P31695 Mouse
- Unigene: 436100 Human
- Unigene: 173813 Mouse
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Alternative names
- FLJ16302 antibody
- hNotch 4 antibody
- hNotch4 antibody
see all
Images
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Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution + Mouse lung lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 210 kDa -
Intracellular flow cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab166605 has been referenced in 3 publications.
- Sabol HM et al. Notch3 signaling between myeloma cells and osteocytes in the tumor niche promotes tumor growth and bone destruction. Neoplasia 28:100785 (2022). PubMed: 35390742
- Sun J et al. HMGA2 regulates CD44 expression to promote gastric cancer cell motility and sphere formation. Am J Cancer Res 7:260-274 (2017). PubMed: 28337375
- Singh NK et al. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine 2:1767-84 (2015). PubMed: 26870802