Overview

  • Product name
    Anti-NOTCH4 antibody [EPNCIR101B]
    See all NOTCH4 primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR101B] to NOTCH4
  • Host species
    Rabbit
  • Specificity
    Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Mouse NOTCH4 aa 1900-2000. The exact sequence is proprietary.

  • Positive control
    • Mouse lung lysate and Jurkat cells
  • General notes

    This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab166605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 210 kDa.

Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.

Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
    • Tissue specificity
      Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the NOTCH family.
      Contains 5 ANK repeats.
      Contains 28 EGF-like domains.
      Contains 3 LNR (Lin/Notch) repeats.
    • Post-translational
      modifications
      Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
      Phosphorylated.
    • Cellular localization
      Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16302 antibody
      • hNotch 4 antibody
      • hNotch4 antibody
      • INT3 antibody
      • Neurogenic locus notch homolog protein 4 antibody
      • NOTC4_HUMAN antibody
      • Notch 4 antibody
      • Notch 4 intracellular domain antibody
      • Notch homolog 4 antibody
      • NOTCH3 antibody
      • Notch4 antibody
      • NOTCH4 protein antibody
      see all

    Images

    • Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution + Mouse lung lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 210 kDa

    • Flow Cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).

    References

    This product has been referenced in:
    • Sun J  et al. HMGA2 regulates CD44 expression to promote gastric cancer cell motility and sphere formation. Am J Cancer Res 7:260-274 (2017). Read more (PubMed: 28337375) »
    • Singh NK  et al. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine 2:1767-84 (2015). Read more (PubMed: 26870802) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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