Product nameAnti-NOTCH4 antibody [EPNCIR101B]
See all NOTCH4 primary antibodies
DescriptionRabbit monoclonal [EPNCIR101B] to NOTCH4
SpecificityData from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Mouse NOTCH4 aa 1900-2000. The exact sequence is proprietary.
- Mouse lung lysate and Jurkat cells
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 210 kDa.
Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
Tissue specificityHighly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
Sequence similaritiesBelongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 28 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Cellular localizationCell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
- Information by UniProt
- FLJ16302 antibody
- hNotch 4 antibody
- hNotch4 antibody
Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution + Mouse lung lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 210 kDa
Flow Cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).
This product has been referenced in:
- Sun J et al. HMGA2 regulates CD44 expression to promote gastric cancer cell motility and sphere formation. Am J Cancer Res 7:260-274 (2017). Read more (PubMed: 28337375) »
- Singh NK et al. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine 2:1767-84 (2015). Read more (PubMed: 26870802) »