Overview

  • Product name

  • Description

    Goat polyclonal to Nova1
  • Host species

    Goat
  • Specificity

    ab77594 is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Nova1 aa 124-137 (internal sequence). Corresponds to NP_002506.2, NP_006480.2, and NP_006482.1.
    Sequence:

    REMPQNVAKTEPVS

  • Positive control

    • WB: Human breast cancer tissue lysate. IHC-P: Human heart and cortex tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab77594 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-Nova1 antibody (ab77594) at 0.01 µg/ml + Human Breast Cancer lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Primary incubation was 1 hour.

  • All lanes : Anti-Nova1 antibody (ab77594) at 0.03 µg/ml

    Lane 1 : Lysate prepared from MOCK transfection.
    Lane 2 : Lysate prepared from transfected HEK293 cells, transiently expressing Nova1

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Primary incubation was 1 hour.

References

This product has been referenced in:

  • Zhang YA  et al. High expression of neuro-oncological ventral antigen 1 correlates with poor prognosis in hepatocellular carcinoma. PLoS One 9:e90955 (2014). Human . Read more (PubMed: 24608171) »
  • Morchikh M  et al. TOX4 and NOVA1 Proteins Are Partners of the LEDGF PWWP Domain and Affect HIV-1 Replication. PLoS One 8:e81217 (2013). WB ; Human . Read more (PubMed: 24312278) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (Any kD (Bio-Rad))
Sample
Mouse Tissue lysate - whole (whole brain)
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 20 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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