Overview

  • Product name

  • Description

    Rabbit polyclonal to Nova1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Xenopus laevis
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 315 and 507 of Human Nova1 (NP_002506).

  • Positive control

    • A431 whole cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • Anti-Nova1 antibody (ab97368) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using Nova1 antibody (ab97368) at 1/200 dilution.

References

This product has been referenced in:

  • Xu J  et al. Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1. MBio 10:N/A (2019). Read more (PubMed: 30696738) »
  • Vernia S  et al. An alternative splicing program promotes adipose tissue thermogenesis. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27635635) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Rat Cell (Primary cortical Neuron)
Specification
Primary cortical Neuron
Permeabilization
Yes - PBS-TrironX100 0,1%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 25 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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