Overview

  • Product name

    Anti-Nova1 antibody [EPR13847]
    See all Nova1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13847] to Nova1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Nova1 aa 1-100. The exact sequence is proprietary.
    Database link: P51513

  • Positive control

    • Mouse brain tissue, PC12 cells, Human cerebellum tissue lysates; Human gliocytoma tissue; Rat brain tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13847
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/250 - 1/500.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Nova1 using ab183024 at 1/500 dilution.  A HRP conjugated Rabbit IgG was used as secondary (prediluted). Counterstain: Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human gliocytoma tissue labeling Nova1 using ab183024 at 1/500 dilution. HRP-conjugated Rabbit IgG was used as secondary (prediluted). Counterstain: Hematoxylin.

  • All lanes : Anti-Nova1 antibody [EPR13847] (ab183024) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : PC12 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Anti-Nova1 antibody [EPR13847] (ab183024) at 1/5000 dilution + Human cerebellum lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 52 kDa

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183024.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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