Product nameNovel Coronavirus IgG Antibody Detection Kit (SARS-CoV-2)
Sample typeSerum, Plasma, Whole Blood
Species reactivityReacts with: Human
Novel Coronavirus IgG Antibody Detection Kit (SARS-CoV-2) (ab272243) is suitable for the qualitative detection of Novel Coronavirus (SARS-CoV-2) IgG antibodies in human serum, plasma, and whole blood.
Note: Novel Coronavirus IgG Antibody (SARS-CoV-2)- ab272243 and Novel Coronavirus IgM Antibody (SARS-CoV-2) - ab272244 are intended for Research Use Only, not for use in diagnostic procedures.
The detection kit uses the principle of immunochromatography: the separation of components in a mixture through a medium using capillary force and the specific and rapid binding of an antibody to its antigen. Each cassette is a dry medium that has been coated separately with novel coronavirus N protein (“T” test line) and goat antichicken IgY antibody (“C” control line) (Figure 1). Two free colloidal gold-labeled antibodies, mouse anti-human IgG (mIgG) and chicken IgY, are in the release pad section (S). Once diluted serum, plasma, or whole blood is applied to the release pad section, the mIgG antibody will bind to coronavirus IgG antibodies if they are present, forming an IgG-IgG complex. The sample and antibodies will then move across the cassette’s medium via capillary action. If coronavirus IgG antibody is present in the sample, the test line (T) will be bound by the IgG-IgG complex and develop color. If there is no coronavirus IgG antibody in the sample, free mIgG will not bind to the test line (T) and no color will develop. The free chicken IgY antibody will bind to the control line (C); this control line should be visible after the detection step as this confirms that the kit is working properly.
The following Detection Kits are available:
Novel Coronavirus IgG Antibody (SARS-CoV-2) - this kit
Novel Coronavirus IgM Antibody (SARS-CoV-2) - ab272244
Notesab272243 and ab272244 were tested on confirmed positive Covid-19 patients sample and known negative samples. The following data set was the outcome of two different screening batches compiled together. Confirmed positive and negative control patients were tested for the presence of IgG and IgM novel coronavirus antibodies. The kit was used as instructed by the manual, and positive or negative indications were listed as such. The results of that testing indicates a sensitivity to viral antibodies of 87%, with a specificity of 84%.
Sample Type Sample IgM- Ab272244 IgG -Ab272243 Overall Positive Overall Negative Sensitivity Specificity Positive 30 24 24 26 4 87% Negative 90 12 3 14 76 84
Storage instructionsStore at +4°C. Please refer to protocols.
Components 20 tests Detection Cassette 20 units Sample Diluent 20 vials
RelevanceCoronavirus specific IgG
Positive for Coronavirus: Both the test line (T) and the quality control line (C) are colored.
Negative for Coronavirus: The test line (T) does not develop color, but the quality control line (C) is colored.
Invalid: There is no colored control line (C) band. The results are invalid regardless of whether a red band appears on the test line (T); additional testing is required.
ΔNote: Regardless of the color saturation present of the band on the test line (T), even a very weak band should be judged as a positive result.
ab272243 has not yet been referenced specifically in any publications.