Overview

  • Product name

  • Description

    Goat polyclonal to NOX1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide:

    DKATDIVTGLKQK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1).

  • Positive control

    • Human and Mouse colon lysates, cultured transgenic Mouse aortic smooth muscle cells (MASM).

Properties

Applications

Our Abpromise guarantee covers the use of ab121009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
  • Tissue specificity

    NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Cellular localization

    Cell projection > invadopodium membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GP91 2 antibody
    • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
    • Mitogenic oxidase 1 antibody
    • MOX-1 antibody
    • MOX1 antibody
    • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
    • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
    • NADPH oxidase 1 antibody
    • NADPH oxidase 1 variant NOH 1L antibody
    • NADPH oxidase homolog 1 antibody
    • NOH 1 antibody
    • NOH-1 antibody
    • NOH1 antibody
    • NOX-1 antibody
    • Nox1 antibody
    • NOX1_HUMAN antibody
    • RP1 146H21.1 antibody
    see all

Images

  • All lanes : Anti-NOX1 antibody (ab121009) at 0.1 µg/ml

    Lane 1 : Colorectal adenocarcinoma cell lysate (35µg protein in RIPA buffer)
    Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer)

    Predicted band size: 65 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

  • All lanes : Anti-NOX1 antibody (ab121009) at 1 µg/ml

    Lane 1 : WT MASM cell lysate (in RIPA buffer)
    Lane 2 : Knock out MASM cell lysate (in RIPA buffer)
    Lane 3 : Transgenic MASM cell lysate (in RIPA buffer)
    Lane 4 : Mouse colon lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 65 kDa

References

This product has been referenced in:

  • De Bessa TC  et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). Read more (PubMed: 30760703) »
  • Alborzinia H  et al. Golgi stress mediates redox imbalance and ferroptosis in human cells. Commun Biol 1:210 (2018). Read more (PubMed: 30511023) »
See all 3 Publications for this product

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