Anti-NOX1 antibody (ab131088)
- Datasheet
- References (14)
- Protocols
Overview
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Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Rabbit polyclonal to NOX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to an internal sequence of Rat NOX1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab131088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
Tissue specificity
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Cellular localization
Cell projection > invadopodium membrane. - Information by UniProt
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Database links
- Entrez Gene: 237038 Mouse
- Entrez Gene: 114243 Rat
- SwissProt: Q8CIZ9 Mouse
- SwissProt: Q9WV87 Rat
- Unigene: 233865 Mouse
- Unigene: 455133 Mouse
- Unigene: 220465 Rat
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Alternative names
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
Images
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Western blot - Anti-NOX1 antibody (ab131088)All lanes : Anti-NOX1 antibody (ab131088)
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lanes 3-4 : Mouse heart tissue lysate
Predicted band size: 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX1 antibody (ab131088)ab131088 staining NOX1 in Rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
References
This product has been referenced in:
- Xu T et al. NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro. Int Immunopharmacol 69:60-70 (2019). Read more (PubMed: 30677569) »
- de Jesus DS et al. Nox1/Ref-1-mediated activation of CREB promotes Gremlin1-driven endothelial cell proliferation and migration. Redox Biol 22:101138 (2019). Read more (PubMed: 30802716) »
