Anti-NOX1 antibody (ab131088)
Key features and details
- Rabbit polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Rabbit polyclonal to NOX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
Synthetic peptide corresponding to an internal sequence of Rat NOX1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Rat
|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.
|
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa. |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
Tissue specificity
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Cellular localization
Cell projection > invadopodium membrane. - Information by UniProt
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Database links
- Entrez Gene: 237038 Mouse
- Entrez Gene: 114243 Rat
- SwissProt: Q8CIZ9 Mouse
- SwissProt: Q9WV87 Rat
- Unigene: 233865 Mouse
- Unigene: 455133 Mouse
- Unigene: 220465 Rat
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Alternative names
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
Images
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Western blot - Anti-NOX1 antibody (ab131088)All lanes : Anti-NOX1 antibody (ab131088)
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lanes 3-4 : Mouse heart tissue lysate
Predicted band size: 65 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX1 antibody (ab131088)ab131088 staining NOX1 in Rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Protocols
References (19)
ab131088 has been referenced in 19 publications.
- Liao CR et al. Advanced oxidation protein products increase TNF-a and IL-1ß expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression. Redox Biol 28:101306 (2020). PubMed: 31539804
- Liu Z et al. IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress. J Exp Clin Cancer Res 39:144 (2020). PubMed: 32727517
- Leger T et al. Dietary EPA Increases Rat Mortality in Diabetes Mellitus, A Phenomenon Which Is Compensated by Green Tea Extract. Antioxidants (Basel) 8:N/A (2019). PubMed: 31690052
- Palacios-Ramírez R et al. Pioglitazone Modulates the Vascular Contractility in Hypertension by Interference with ET-1 Pathway. Sci Rep 9:16461 (2019). PubMed: 31712626
- Xu T et al. NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro. Int Immunopharmacol 69:60-70 (2019). PubMed: 30677569
