Overview

  • Product name

    Anti-NOX2/gp91phox antibody
    See all NOX2/gp91phox primary antibodies
  • Description

    Rabbit polyclonal to NOX2/gp91phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids of Human NOX2.

  • Positive control

    • HeLa, Jurkat, MCF7 and SMMC cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab131083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
  • Involvement in disease

    Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMCBX2 antibody
    • CGD antibody
    • CGD91-phox antibody
    • CY24B_HUMAN antibody
    • CYBB antibody
    • Cytochrome b 245, beta polypeptide antibody
    • Cytochrome b(558) beta chain antibody
    • Cytochrome b(558) subunit beta antibody
    • Cytochrome b-245 heavy chain antibody
    • Cytochrome b558 subunit beta antibody
    • GP91 PHOX antibody
    • gp91-1 antibody
    • gp91-phox antibody
    • GP91PHOX antibody
    • Heme-binding membrane glycoprotein gp91phox antibody
    • NADPH oxidase 2 antibody
    • Neutrophil cytochrome b 91 kDa polypeptide antibody
    • NOX2 antibody
    • p22 phagocyte B-cytochrome antibody
    • P91 PHOX antibody
    • p91-PHOX antibody
    • Superoxide-generating NADPH oxidase heavy chain subunit antibody
    see all

Images

  • All lanes : Anti-NOX2/gp91phox antibody (ab131083) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : SMMC cell lysate

References

This product has been referenced in:

  • Wang D  et al. The Sodium-Glucose Cotransporter 2 Inhibitor Dapagliflozin Prevents Renal and Liver Disease in Western Diet Induced Obesity Mice. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29301371) »
  • Dyugovskaya L  et al. Intermittent Hypoxia Affects the Spontaneous Differentiation In Vitro of Human Neutrophils into Long-Lived Giant Phagocytes. Oxid Med Cell Longev 2016:9636937 (2016). Read more (PubMed: 26635914) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

I have had a look into this and there has been an error on our part. The antibody ab131083 was assigned the incorrect target (DUOX2) when it is actually raised against human NOX2. This is why the Western blot does not correspond to the DUOX2 predicted size. We have now amended this. Thank you for pointing this out to us. We have made all customers who have purchased this antibody aware of this.

We do have an alternative antibody raised against DUOX2. This is the rabbit polyclonal ab65813. This has been used in Western blotting with rat and human samples. If you would like to try the antibody in any other applications or species of samples you may be eligible for the Abtrial testing scheme. More information on this can be found from the following link:

https://www.abcam.com/abtrial

If you would be interested in taking part in this scheme please let me know how you intend to use the antibody.

I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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