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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NOX2/gp91phox.
(Peptide available as ab32458.)
Our Abpromise guarantee covers the use of ab31092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22184405|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).|
Cow aortic endothelial cells stained for NOX2/gp91phox (green) using ab31092 (1/100 dilution) in ICC/IF. Cells were permeabilized in 0.1% saponin and fixed in 4% paraformaldehyde.
ab31092 at a 1/2000 dilution staining NOX2/gp91phox in paraffin-embedded human tonsil tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.
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