Recombinant Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-250037-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg "Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6991] to NOX2/gp91phox
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NOX2/gp91phox antibody [EPR6991]
See all NOX2/gp91phox primary antibodies -
Description
Rabbit monoclonal [EPR6991] to NOX2/gp91phox -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.70 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6991 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Mouse spleen tissue lysate - total protein (ab29293)
- Caco-2 whole cell lysate (ab3950)
- Mouse brain tissue lysate - total protein (ab4022)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse spleen tissue lysate - total protein (14 days) (ab7274)
- Mouse spleen tissue lysate - total protein (7 days) (ab7275)
- Mouse spleen tissue lysate - total protein (ab7937)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
For unpurified use at 1/1000 - 1/10000. |
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). For unpurified use at 1/1000 - 1/10000. |
Target
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Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. -
Involvement in disease
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1536 Human
- Entrez Gene: 13058 Mouse
- Entrez Gene: 66021 Rat
- Omim: 300481 Human
- SwissProt: P04839 Human
- SwissProt: Q61093 Mouse
- Unigene: 292356 Human
- Unigene: 200362 Mouse
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Alternative names
- AMCBX2 antibody
- CGD antibody
- CGD91-phox antibody
see all
Images
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Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-250036-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg)
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-143006-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg)
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-143005-anti-nox2-gp91phox-antibody-epr6991.jpg)
OI-RD Scanning - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) -
![Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-6-benefits-of-recombinant-antibodies.png)
Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab129068 has been referenced in 61 publications.
- Meng K et al. The Long Noncoding RNA Hotair Regulates Oxidative Stress and Cardiac Myocyte Apoptosis during Ischemia-Reperfusion Injury. Oxid Med Cell Longev 2020:1645249 (2020). PubMed: 32256945
- Wang ZP et al. Corosolic acid attenuates cardiac fibrosis following myocardial infarction in mice. Int J Mol Med 45:1425-1435 (2020). PubMed: 32323841
- Chang Z et al. Protective effects of isorhamnetin on pulmonary arterial hypertension: in vivo and in vitro studies. Phytother Res N/A:N/A (2020). PubMed: 32452118
- Lin CC et al. Induction of HO-1 by Mevastatin Mediated via a Nox/ROS-Dependent c-Src/PDGFRa/PI3K/Akt/Nrf2/ARE Cascade Suppresses TNF-a-Induced Lung Inflammation. J Clin Med 9:N/A (2020). PubMed: 31952230
- Zhang J et al. DNase I improves corneal epithelial and nerve regeneration in diabetic mice. J Cell Mol Med 24:4547-4556 (2020). PubMed: 32168430