Recombinant
Recombinant Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
- Datasheet
- References (46)
- Protocols
Overview
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Product name
Anti-NOX2/gp91phox antibody [EPR6991]
See all NOX2/gp91phox primary antibodies -
Description
Rabbit monoclonal [EPR6991] to NOX2/gp91phox -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.70 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6991 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Mouse spleen tissue lysate - total protein (ab29293)
- MCF7 whole cell lysate (ab3871)
- Caco 2 whole cell lysate (ab3950)
- Mouse brain tissue lysate - total protein (ab4022)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse spleen tissue lysate - total protein (14 days) (ab7274)
- Mouse spleen tissue lysate - total protein (7 days) (ab7275)
- HepG2 whole cell lysate (ab7900)
- Mouse spleen tissue lysate - total protein (ab7937)
Applications
Our Abpromise guarantee covers the use of ab129068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). For unpurified use at 1/1000 - 1/10000. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. -
Involvement in disease
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1536 Human
- Entrez Gene: 13058 Mouse
- Entrez Gene: 66021 Rat
- Omim: 300481 Human
- SwissProt: P04839 Human
- SwissProt: Q61093 Mouse
- Unigene: 292356 Human
- Unigene: 200362 Mouse
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Alternative names
- AMCBX2 antibody
- CGD antibody
- CGD91-phox antibody
see all
Images
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![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-250036-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg)
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-250037-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg)
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-143006-anti-nox2-gp91phox-antibody-epr6991-western-blot.jpg)
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Other - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)](https://www.abcam.com/ps/products/129/ab129068/Images/ab129068-143005-anti-nox2-gp91phox-antibody-epr6991.jpg)
Other - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
Protocols
References
This product has been referenced in:
- Li Z et al. Protective effects of tetramethylpyrazine analogue Z-11 on cerebral ischemia reperfusion injury. Eur J Pharmacol 844:156-164 (2019). Read more (PubMed: 30502344) »
- Chen HH et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). Read more (PubMed: 30668894) »
