Overview

  • Product name

    Anti-NOX2/gp91phox antibody [EPR6991]
    See all NOX2/gp91phox primary antibodies
  • Description

    Rabbit monoclonal [EPR6991] to NOX2/gp91phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
    • Involvement in disease

      Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similarities

      Contains 1 FAD-binding FR-type domain.
      Contains 1 ferric oxidoreductase domain.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMCBX2 antibody
      • CGD antibody
      • CGD91-phox antibody
      • CY24B_HUMAN antibody
      • CYBB antibody
      • Cytochrome b 245, beta polypeptide antibody
      • Cytochrome b(558) beta chain antibody
      • Cytochrome b(558) subunit beta antibody
      • Cytochrome b-245 heavy chain antibody
      • Cytochrome b558 subunit beta antibody
      • GP91 PHOX antibody
      • gp91-1 antibody
      • gp91-phox antibody
      • GP91PHOX antibody
      • Heme-binding membrane glycoprotein gp91phox antibody
      • NADPH oxidase 2 antibody
      • Neutrophil cytochrome b 91 kDa polypeptide antibody
      • NOX2 antibody
      • p22 phagocyte B-cytochrome antibody
      • P91 PHOX antibody
      • p91-PHOX antibody
      • Superoxide-generating NADPH oxidase heavy chain subunit antibody
      see all

    Images

    • All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse spleen tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Li Z  et al. Protective effects of tetramethylpyrazine analogue Z-11 on cerebral ischemia reperfusion injury. Eur J Pharmacol 844:156-164 (2019). Read more (PubMed: 30502344) »
    • Chen HH  et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). Read more (PubMed: 30668894) »
    See all 41 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Nov 28 2017

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (9%)
    Loading amount
    60 µg
    Treatment
    TNF-a 30min
    Specification
    heart
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Mr. Baptiste Jude

    Verified customer

    Submitted Jul 06 2016

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Dermal fibroblast)
    Specification
    Dermal fibroblast
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Karina Ricart

    Verified customer

    Submitted Jul 31 2014

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (EA. hy926)
    Loading amount
    10 µg
    Specification
    EA. hy926
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jan 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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