Name: Anti-NOX2/gp91phox antibody [EPR6991]
Brand: RabMAb
SKU: ab129068
Rating: 5 (4 reviews)

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Primary

Secondary

This product Rabbit Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)
WB

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-NOX2/gp91phox antibody [EPR6991]
See all NOX2/gp91phox primary antibodies
Description

Rabbit monoclonal [EPR6991] to NOX2/gp91phox
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.
Positive control
- WB: MCF-7, HepG2 and Caco-2 cell lysates, mouse and rat brain and spleen tissue lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.70 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6991
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab129068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa). For unpurified use at 1/1000 - 1/10000.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Function

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Involvement in disease

Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similarities

Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
Post-translational
modifications

Glycosylated.
Cellular localization

Membrane.
Target information above from: UniProt accession P04839 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1536 Human
- Entrez Gene: 13058 Mouse
- Entrez Gene: 66021 Rat
- Omim: 300481 Human
- SwissProt: P04839 Human
- SwissProt: Q61093 Mouse
- Unigene: 292356 Human
- Unigene: 200362 Mouse
Alternative names
- AMCBX2 antibody
- CGD antibody
- CGD91-phox antibody
- CY24B_HUMAN antibody
- CYBB antibody
- Cytochrome b 245, beta polypeptide antibody
- Cytochrome b(558) beta chain antibody
- Cytochrome b(558) subunit beta antibody
- Cytochrome b-245 heavy chain antibody
- Cytochrome b558 subunit beta antibody
- GP91 PHOX antibody
- gp91-1 antibody
- gp91-phox antibody
- GP91PHOX antibody
- Heme-binding membrane glycoprotein gp91phox antibody
- NADPH oxidase 2 antibody
- Neutrophil cytochrome b 91 kDa polypeptide antibody
- NOX2 antibody
- p22 phagocyte B-cytochrome antibody
- P91 PHOX antibody
- p91-PHOX antibody
- Superoxide-generating NADPH oxidase heavy chain subunit antibody
see all

Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 65 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/1000 dilution (unpurified)

Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 65 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Other - Anti-NOX2/gp91phox antibody [EPR6991] (ab129068)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Western blot protocols

This product has been referenced in:

Li Z et al. Protective effects of tetramethylpyrazine analogue Z-11 on cerebral ischemia reperfusion injury. Eur J Pharmacol 844:156-164 (2019). Read more (PubMed: 30502344) »
Chen HH et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). Read more (PubMed: 30668894) »

See all 41 Publications for this product

Publishing research using ab129068? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Pig Tissue lysate - whole (Kidney)

Gel Running Conditions

Reduced Denaturing

Loading amount

35 µg

Specification

Kidney

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 28 2017

Application

Western blot

Sample

Rat Tissue lysate - whole (heart)

Gel Running Conditions

Reduced Denaturing (9%)

Loading amount

60 µg

Treatment

TNF-a 30min

Specification

heart

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Baptiste Jude

Verified customer

Submitted Jul 06 2016

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing

Sample

Human Cell lysate - whole cell (Dermal fibroblast)

Specification

Dermal fibroblast

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Karina Ricart

Verified customer

Submitted Jul 31 2014

Application

Western blot

Sample

Human Cell lysate - whole cell (EA. hy926)

Loading amount

10 µg

Specification

EA. hy926

Gel Running Conditions

Non-reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

Western blot abreview for Anti-NOX2/gp91phox antibody [EPR6991]

Dissociation constant (K_D)

Post-translational
modifications