Recombinant
RabMAb

Recombinant Anti-NOX2/gp91phox antibody [EPR6991] - BSA and Azide free (ab238964)

Overview

  • Product name

    Anti-NOX2/gp91phox antibody [EPR6991] - BSA and Azide free
    See all NOX2/gp91phox primary antibodies
  • Description

    Rabbit monoclonal [EPR6991] to NOX2/gp91phox - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NOX2/gp91phox aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: MCF7, HepG2 and Caco-2 cell lysate.
  • General notes

    Ab238964 is the carrier-free version of ab129068. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238964 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
    • Involvement in disease

      Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similarities

      Contains 1 FAD-binding FR-type domain.
      Contains 1 ferric oxidoreductase domain.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMCBX2 antibody
      • CGD antibody
      • CGD91-phox antibody
      • CY24B_HUMAN antibody
      • CYBB antibody
      • Cytochrome b 245, beta polypeptide antibody
      • Cytochrome b(558) beta chain antibody
      • Cytochrome b(558) subunit beta antibody
      • Cytochrome b-245 heavy chain antibody
      • Cytochrome b558 subunit beta antibody
      • GP91 PHOX antibody
      • gp91-1 antibody
      • gp91-phox antibody
      • GP91PHOX antibody
      • Heme-binding membrane glycoprotein gp91phox antibody
      • NADPH oxidase 2 antibody
      • Neutrophil cytochrome b 91 kDa polypeptide antibody
      • NOX2 antibody
      • p22 phagocyte B-cytochrome antibody
      • P91 PHOX antibody
      • p91-PHOX antibody
      • Superoxide-generating NADPH oxidase heavy chain subunit antibody
      see all

    Images

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129068).

    • All lanes : Anti-NOX2/gp91phox antibody [EPR6991] (ab129068) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 65 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab129068).

    References

    ab238964 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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