Overview

  • Product name

  • Description

    Rabbit polyclonal to NOX5
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total NOX5 protein.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Fusion protein (GST-tag) corresponding to Human NOX5 (C terminal).
    Database link: Q96PH1

  • Positive control

    • 293T and HeLa cell, human testis tissue, skov3, Raji and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab198213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 86 kDa.

Target

  • Function

    Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis.
  • Tissue specificity

    Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform 3 is expressed in spleen. Isoform 4 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells.
  • Sequence similarities

    Contains 4 EF-hand domains.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Developmental stage

    Expressed in fetal tissues.
  • Domain

    Isoform 3 and isoform 4 of this protein have four functional EF-hand calcium-binding domains. Isoform 1 and isoform 2 have the third EF-hand domain interrupted by an insert.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC149776 antibody
    • MGC149777 antibody
    • NADPH oxidase 5 antibody
    • NADPH oxidase EFhand calcium binding domain 5 antibody
    • NOX5 antibody
    • NOX5_HUMAN antibody
    see all

Images

  • All lanes : Anti-NOX5 antibody (ab198213) at 1/200 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human testis tissue cell lysate
    Lane 4 : Skov3 cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : Jurkat cell lysate

    Developed using the ECL technique.

    Predicted band size: 86 kDa


    Exposure time: 20 seconds


    Gel: 6% SDS-PAGE

References

This product has been referenced in:

  • Du S  et al. NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR. Cell Death Dis 9:948 (2018). Read more (PubMed: 30237423) »
See 1 Publication for this product

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