Key features and details
- Rabbit polyclonal to NOX5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NOX5 antibody
See all NOX5 primary antibodies
DescriptionRabbit polyclonal to NOX5
SpecificityThis antibody detects endogenous levels of total NOX5 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Fusion protein (GST-tag) corresponding to Human NOX5 (C terminal).
Database link: Q96PH1
- 293T and HeLa cell, human testis tissue, skov3, Raji and Jurkat cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 86 kDa.|
FunctionCalcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis.
Tissue specificityMainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform 3 is expressed in spleen. Isoform 4 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells.
Sequence similaritiesContains 4 EF-hand domains.
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
Developmental stageExpressed in fetal tissues.
DomainIsoform 3 and isoform 4 of this protein have four functional EF-hand calcium-binding domains. Isoform 1 and isoform 2 have the third EF-hand domain interrupted by an insert.
- Information by UniProt
- MGC149776 antibody
- MGC149777 antibody
- NADPH oxidase 5 antibody
All lanes : Anti-NOX5 antibody (ab198213) at 1/200 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human testis tissue cell lysate
Lane 4 : Skov3 cell lysate
Lane 5 : Raji cell lysate
Lane 6 : Jurkat cell lysate
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 20 seconds
Gel: 6% SDS-PAGE
ab198213 has been referenced in 1 publication.
- Du S et al. NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR. Cell Death Dis 9:948 (2018). PubMed: 30237423