Anti-Noxa antibody (ab23563)
Key features and details
- Rabbit polyclonal to Noxa
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Noxa antibody
See all Noxa primary antibodies -
Description
Rabbit polyclonal to Noxa -
Host species
Rabbit -
Specificity
ab23563 recognises a 16.5kDa Noxa molecule. Western Blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum, -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide:
CAQLRRIGDKVNLRQK
, corresponding to C terminal amino acids 75/90 of Mouse Noxa, coupled to Diphtheria Toxoid via a cysteine molecule added at the amino terminal of the peptide sequence. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
Target
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Function
Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. -
Tissue specificity
Highly expressed in adult T-cell leukemia cell line. -
Sequence similarities
Belongs to the PMAIP1 family. -
Domain
The BH3 motif is essential for pro-apoptotic activity. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 58801 Mouse
- SwissProt: Q9JM54 Mouse
- Unigene: 271878 Mouse
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Alternative names
- Adult T cell leukemia derived PMA responsive antibody
- APR antibody
- APR_HUMAN antibody
see all
Datasheets and documents
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Datasheet download
References (3)
ab23563 has been referenced in 3 publications.
- Zhou Z et al. Betulin induces cytochrome c release and apoptosis in colon cancer cells via NOXA. Oncol Lett 15:7319-7327 (2018). PubMed: 29725447
- Pfeiffer S et al. Analysis of BH3-only proteins upregulated in response to oxygen/glucose deprivation in cortical neurons identifies Bmf but not Noxa as potential mediator of neuronal injury. Cell Death Dis 5:e1456 (2014). WB ; Mouse . PubMed: 25299781
- Lainey E et al. Azacytidine and erlotinib exert synergistic effects against acute myeloid leukemia. Oncogene : (2012). PubMed: 23085751
