Anti-Noxa antibody (ab23563)

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Overview

  • Product name
    Anti-Noxa antibody
    See all Noxa primary antibodies
  • Description
    Rabbit polyclonal to Noxa
  • Host species
    Rabbit
  • Specificity
    ab23563 recognises a 16.5kDa Noxa molecule. Western Blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum,
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    CAQLRRIGDKVNLRQK

    , corresponding to C terminal amino acids 75/90 of Mouse Noxa, coupled to Diphtheria Toxoid via a cysteine molecule added at the amino terminal of the peptide sequence.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab23563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 16 kDa.

Target

  • Function
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • Tissue specificity
    Highly expressed in adult T-cell leukemia cell line.
  • Sequence similarities
    Belongs to the PMAIP1 family.
  • Domain
    The BH3 motif is essential for pro-apoptotic activity.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    • Alternative names
      • Adult T cell leukemia derived PMA responsive antibody
      • APR antibody
      • APR_HUMAN antibody
      • ATL-derived antibody
      • Immediate early response protein APR antibody
      • Immediate-early-response protein APR antibody
      • NOXA antibody
      • Phorbol 12 myristate 13 acetate induced protein 1 antibody
      • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
      • PMA induced protein 1 antibody
      • PMA-induced protein 1 antibody
      • PMA-responsive gene antibody
      • Pmaip1 antibody
      • Protein Noxa antibody
      see all

    References

    This product has been referenced in:
    • Zhou Z  et al. Betulin induces cytochrome c release and apoptosis in colon cancer cells via NOXA. Oncol Lett 15:7319-7327 (2018). Read more (PubMed: 29725447) »
    • Pfeiffer S  et al. Analysis of BH3-only proteins upregulated in response to oxygen/glucose deprivation in cortical neurons identifies Bmf but not Noxa as potential mediator of neuronal injury. Cell Death Dis 5:e1456 (2014). WB ; Mouse . Read more (PubMed: 25299781) »
    See all 3 Publications for this product

    Publishing research using ab23563? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Noxa antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Primary cortical neurons)
    Permeabilization
    Yes - 0,1% Triton in PBS
    Specification
    Primary cortical neurons
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 02 2016

    Flow Cytometry abreview for Anti-Noxa antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Primary mouse neurons)
    Permeabilization
    Yes - Triton 0.2%
    Gating Strategy
    Live and dead cells
    Specification
    Primary mouse neurons
    Preparation
    Cell harvesting/tissue preparation method: (1200rpm, 5' at 4ºC) serial centrifugation
    Sample buffer: PBS-BSA 1%
    Fixation
    Paraformaldehyde
    Read More

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 02 2016

    Western blot abreview for Anti-Noxa antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (14)
    Sample
    Mouse Cell lysate - whole cell (Primary neurons as well as spleen/brain tissue)
    Specification
    Primary neurons as well as spleen/brain tissue
    Treatment
    Oxygen/glucose deprivation
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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