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  1. Link

    noxa-antibody-ab23563.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-Noxa antibody (ab23563)

  • Datasheet
Reviews (3) Submit a question References (3)

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Key features and details

  • Rabbit polyclonal to Noxa
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

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Protein
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Recombinant Human Noxa protein (ab152618)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Noxa antibody
    See all Noxa primary antibodies
  • Description

    Rabbit polyclonal to Noxa
  • Host species

    Rabbit
  • Specificity

    ab23563 recognises a 16.5kDa Noxa molecule. Western Blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum,
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    CAQLRRIGDKVNLRQK

    , corresponding to C terminal amino acids 75/90 of Mouse Noxa, coupled to Diphtheria Toxoid via a cysteine molecule added at the amino terminal of the peptide sequence.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Noxa protein (ab152618)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.

Target

  • Function

    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • Tissue specificity

    Highly expressed in adult T-cell leukemia cell line.
  • Sequence similarities

    Belongs to the PMAIP1 family.
  • Domain

    The BH3 motif is essential for pro-apoptotic activity.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q13794 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58801 Mouse
    • SwissProt: Q9JM54 Mouse
    • Unigene: 271878 Mouse
    • Alternative names

      • Adult T cell leukemia derived PMA responsive antibody
      • APR antibody
      • APR_HUMAN antibody
      • ATL-derived antibody
      • Immediate early response protein APR antibody
      • Immediate-early-response protein APR antibody
      • NOXA antibody
      • Phorbol 12 myristate 13 acetate induced protein 1 antibody
      • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
      • PMA induced protein 1 antibody
      • PMA-induced protein 1 antibody
      • PMA-responsive gene antibody
      • Pmaip1 antibody
      • Protein Noxa antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab23563? Please let us know so that we can cite the reference in this datasheet.

    ab23563 has been referenced in 3 publications.

    • Zhou Z  et al. Betulin induces cytochrome c release and apoptosis in colon cancer cells via NOXA. Oncol Lett 15:7319-7327 (2018). PubMed: 29725447
    • Pfeiffer S  et al. Analysis of BH3-only proteins upregulated in response to oxygen/glucose deprivation in cortical neurons identifies Bmf but not Noxa as potential mediator of neuronal injury. Cell Death Dis 5:e1456 (2014). WB ; Mouse . PubMed: 25299781
    • Lainey E  et al. Azacytidine and erlotinib exert synergistic effects against acute myeloid leukemia. Oncogene : (2012). PubMed: 23085751

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Noxa antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Primary cortical neurons)
    Permeabilization
    Yes - 0,1% Triton in PBS
    Specification
    Primary cortical neurons
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 02 2016

    Flow Cytometry abreview for Anti-Noxa antibody

    Poor
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Primary mouse neurons)
    Permeabilization
    Yes - Triton 0.2%
    Gating Strategy
    Live and dead cells
    Specification
    Primary mouse neurons
    Preparation
    Cell harvesting/tissue preparation method: (1200rpm, 5' at 4ºC) serial centrifugation
    Sample buffer: PBS-BSA 1%
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 02 2016

    Western blot abreview for Anti-Noxa antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (14)
    Sample
    Mouse Cell lysate - whole cell (Primary neurons as well as spleen/brain tissue)
    Specification
    Primary neurons as well as spleen/brain tissue
    Treatment
    Oxygen/glucose deprivation
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Cristina Muñoz-Pinedo

    Verified customer

    Submitted Feb 11 2015

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