Key features and details
- Rabbit polyclonal to NOXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NOXA1 antibody
See all NOXA1 primary antibodies
DescriptionRabbit polyclonal to NOXA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human NOXA1 aa 150-313.
Database link: Q86UR1
- WB: Mouse brain lysate. IHC: Human lung cancer and kidney tissues. ICC/IF: HepG2 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionFunctions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.
Tissue specificityWidely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon.
Sequence similaritiesBelongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 1 SH3 domain.
Contains 4 TPR repeats.
Developmental stageExpressed in fetal kidney.
DomainThe SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
The TPR repeats mediate interaction with RAC1.
modificationsInteraction with YWHAZ depends on phosphorylation by PKA.
Cellular localizationCytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.
- Information by UniProt
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Paraffin-embedded human lung cancer tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.
Anti-NOXA1 antibody (ab222852) at 1/1000 dilution + Mouse brain lysate
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Paraffin-embedded human kidney tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for NOXA1 (green) using ab222852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
ab222852 has not yet been referenced specifically in any publications.