Overview

  • Product name

  • Description

    Rabbit polyclonal to NOXA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human NOXA1 aa 150-313.
    Database link: Q86UR1

  • Positive control

    • WB: Mouse brain lysate. IHC: Human lung cancer and kidney tissues. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.
  • Tissue specificity

    Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon.
  • Sequence similarities

    Belongs to the NCF2/NOXA1 family.
    Contains 1 OPR domain.
    Contains 1 SH3 domain.
    Contains 4 TPR repeats.
  • Developmental stage

    Expressed in fetal kidney.
  • Domain

    The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
    The TPR repeats mediate interaction with RAC1.
  • Post-translational
    modifications

    Interaction with YWHAZ depends on phosphorylation by PKA.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 31 antibody
    • Antigen NY-CO-31 antibody
    • FLJ25475 antibody
    • Inhibitory NADPH oxidase activator 1 antibody
    • MGC131800 antibody
    • NADPH oxidase activator 1 antibody
    • NCF2 like protein antibody
    • NCF2-like protein antibody
    • NOX activator 1 antibody
    • NOXA 1 antibody
    • Noxa1 antibody
    • NOXA1_HUMAN antibody
    • NY CO 31 antibody
    • p51 nox antibody
    • p51-nox antibody
    • p51NOX antibody
    • P67phox like factor antibody
    • P67phox-like factor antibody
    • SDCCAG31 antibody
    • Serologically defined colon cancer antigen 31 antibody
    see all

Images

  • Paraffin-embedded human lung cancer tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.

  • Anti-NOXA1 antibody (ab222852) at 1/1000 dilution + Mouse brain lysate

    Secondary
    Goat polyclonal to rabbit at 1/10000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Paraffin-embedded human kidney tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for NOXA1 (green) using ab222852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

References

ab222852 has not yet been referenced specifically in any publications.

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