Anti-NOXA1 antibody (ab68523)
Key features and details
- Mouse polyclonal to NOXA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOXA1 antibody
See all NOXA1 primary antibodies -
Description
Mouse polyclonal to NOXA1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human NOXA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- NOXA1 transfected 293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. -
Tissue specificity
Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. -
Sequence similarities
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 1 SH3 domain.
Contains 4 TPR repeats. -
Developmental stage
Expressed in fetal kidney. -
Domain
The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
The TPR repeats mediate interaction with RAC1. -
Post-translational
modificationsInteraction with YWHAZ depends on phosphorylation by PKA. -
Cellular localization
Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1. - Information by UniProt
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Database links
- Entrez Gene: 10811 Human
- Omim: 611255 Human
- SwissProt: Q86UR1 Human
- Unigene: 495554 Human
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Alternative names
- Antigen NY CO 31 antibody
- Antigen NY-CO-31 antibody
- FLJ25475 antibody
see all
Images
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All lanes : Anti-NOXA1 antibody (ab68523) at 1/500 dilution
Lane 1 : NOXA1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (5)
ab68523 has been referenced in 5 publications.
- De Bessa TC et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). PubMed: 30760703
- Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget 8:103710-103730 (2017). PubMed: 29262595
- Streeter J et al. Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circ Res 115:911-8 (2014). PubMed: 25228390
- Csányi G et al. Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med 51:1116-25 (2011). WB ; Human . PubMed: 21586323
- Biasi F et al. Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radic Biol Med 47:1731-41 (2009). IP ; Human . PubMed: 19778604