Key features and details
- Rabbit polyclonal to NOXA2/p67phox
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NOXA2/p67phox antibody
See all NOXA2/p67phox primary antibodies
DescriptionRabbit polyclonal to NOXA2/p67phox
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human NOXA2/p67phox aa 1-526.
MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTIL KNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQ LRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSM KSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLG KATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFG FVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELR IHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPM PYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSN ELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQ TTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECK GKVGIFPKVFVEDCATTDLESTRREV
Database link: P19878
- THP1 cell and spleen cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 60 kDa.|
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesBelongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 2 SH3 domains.
Contains 3 TPR repeats.
- Information by UniProt
- 67 kDa neutrophil oxidase factor antibody
- Chronic granulomatous disease autosomal 2 antibody
- FLJ93058 antibody
All lanes : Anti-NOXA2/p67phox antibody (ab175293) at 1/500 dilution
Lane 1 : THP1 cell lysate
Lane 2 : mouse spleen cell lysate
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling NOXA2/p67phox with ab175293 at 1/100. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling NOXA2/p67phox with ab175293 at 1/100. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175293. Blue DAPI for nuclear staining.
ab175293 has not yet been referenced specifically in any publications.