Recombinant
RabMAb

Recombinant Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)

Overview

  • Product name

    Anti-NOXA2/p67phox antibody [EPR5064]
    See all NOXA2/p67phox primary antibodies
  • Description

    Rabbit monoclonal [EPR5064] to NOXA2/p67phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. within Human NOXA2/p67phox aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: P19878

  • Positive control

    • Human spleen tissue. THP-1 cell lysate. HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/500 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • Involvement in disease

      Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similarities

      Belongs to the NCF2/NOXA1 family.
      Contains 1 OPR domain.
      Contains 2 SH3 domains.
      Contains 3 TPR repeats.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 67 kDa neutrophil oxidase factor antibody
      • Chronic granulomatous disease autosomal 2 antibody
      • FLJ93058 antibody
      • NADPH oxidase activator 2 antibody
      • NCF-2 antibody
      • Ncf2 antibody
      • NCF2_HUMAN antibody
      • Neutrophil cytosol factor 2 antibody
      • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
      • Neutrophil NADPH oxidase factor 2 antibody
      • NOXA2 antibody
      • P67 PHOX antibody
      • p67-phox antibody
      • p67phox antibody
      see all

    Images

    • All lanes : Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution

      Lane 1 : HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA
      Lane 2 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Chen HH  et al. Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats. Mol Nutr Food Res 63:e1801042 (2019). Read more (PubMed: 30668894) »
    • Zhang Y  et al. YiQiFuMai Powder Injection Attenuates Coronary Artery Ligation-Induced Heart Failure Through Improving Mitochondrial Function via Regulating ROS Generation and CaMKII Signaling Pathways. Front Pharmacol 10:381 (2019). Read more (PubMed: 31031629) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1 cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    THP-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jun 12 2017

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 01 2016

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 01 2016

    Answer

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