Product nameAnti-NOXA2/p67phox antibody [EPR5065]
See all NOXA2/p67phox primary antibodies
DescriptionRabbit monoclonal [EPR5065] to NOXA2/p67phox
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human NOXA2/p67phox aa 400-500. The exact sequence is proprietary.
- THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/500 - 1/1000.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesBelongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 2 SH3 domains.
Contains 3 TPR repeats.
- Information by UniProt
- 67 kDa neutrophil oxidase factor antibody
- Chronic granulomatous disease autosomal 2 antibody
- FLJ93058 antibody
All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1:150. The secondary antibody is a goat anti-rabbit IgG FITC.