The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20000 - 1/100000.
1/2000 - 1/8000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.
Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.
The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation. The SH3 domains mediate interaction with CYBA/p22phox. Also mediates intramolecular interaction with the proline-rich region.
Cell membrane. Isoform 3 associates with the plasma membrane in a lipid-dependent manner.
Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget8:103710-103730 (2017).
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