Overview

  • Product name

  • Description

    Rabbit polyclonal to NPAS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 549-816 of Human NPAS2.

  • Positive control

    • H1299, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells; H441 xenograft; A431, Molt-4 and Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab137601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    BMAL1-NPAS2 heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix loop helix PAS protein MOP4 antibody
    • Basic-helix-loop-helix-PAS protein MOP4 antibody
    • bHLHe9 antibody
    • class E basic helix loop helix protein 9 antibody
    • Class E basic helix-loop-helix protein 9 antibody
    • FLJ23138 antibody
    • Member of PAS protein 4 antibody
    • Member of PAS superfamily 4 antibody
    • MGC71151 antibody
    • MOP4 antibody
    • Neuronal PAS domain containing protein 2 antibody
    • Neuronal PAS domain protein 2 antibody
    • Neuronal PAS domain-containing protein 2 antibody
    • Neuronal PAS2 antibody
    • NPAS2 antibody
    • NPAS2_HUMAN antibody
    • PAS domain containing protein 4 antibody
    • PAS domain-containing protein 4 antibody
    • PASD4 antibody
    see all

Images

  • All lanes : Anti-NPAS2 antibody (ab137601) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate
    Lane 3 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 91 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling NPAS2 with ab137601 at 1/200 dilution. Lower panel is costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded H441 xenograft labelling NPAS2 with ab137601 at 1/100 dilution.

References

This product has been referenced in:

  • Ramasamy R  et al. Whole-exome sequencing identifies novel homozygous mutation in NPAS2 in family with nonobstructive azoospermia. Fertil Steril 104:286-91 (2015). Read more (PubMed: 25956372) »
See 1 Publication for this product

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