Key features and details
- Mouse monoclonal [S408-79] to Npas4
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-Npas4 antibody [S408-79]
See all Npas4 primary antibodies
DescriptionMouse monoclonal [S408-79] to Npas4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein corresponding to Rat Npas4 aa 597-802.
PLSVDVPLVPEGLLTPEASPVKQSFFHYTEKEQNEIDRLIQQISQLAQGM DRPFSAEAGTGGLEPLGGLEPLNPNLSLSGAGPPVLSLDLKPWKCQELDF LVDPDNLFLEETPVEDIFMDLSTPDPNGEWGSGDPEAEVPGGTLSPCNNL SPEDHSFLEDLATYETAFETGVSTFPYEGFADELHQLQSQVQDSFHEDGS GGEPTF
Database link: Q8CJH6
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab242003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 87 kDa.|
FunctionActs as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
- Information by UniProt
- bHLH PAS type transcription factor NXF antibody
- bHLHe79 antibody
- Class E basic helix-loop-helix protein 79 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Npas4 (green, C) using ab242003 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 1 hr at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
Anti-Npas4 antibody [S408-79] (ab242003) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 6 minutes
Primary incubation for 16 hours at 4°C.
Secondary incubatin for 1 hr at RT.
Blocked for 1 hr at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242003 has not yet been referenced specifically in any publications.