Product nameAnti-Npas4 antibody [S408-79]
See all Npas4 primary antibodies
DescriptionMouse monoclonal [S408-79] to Npas4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein corresponding to Rat Npas4 aa 597-802.
PLSVDVPLVPEGLLTPEASPVKQSFFHYTEKEQNEIDRLIQQISQLAQGM DRPFSAEAGTGGLEPLGGLEPLNPNLSLSGAGPPVLSLDLKPWKCQELDF LVDPDNLFLEETPVEDIFMDLSTPDPNGEWGSGDPEAEVPGGTLSPCNNL SPEDHSFLEDLATYETAFETGVSTFPYEGFADELHQLQSQVQDSFHEDGS GGEPTF
Database link: Q8CJH6
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab242003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 87 kDa.|
FunctionActs as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
- Information by UniProt
- bHLH PAS type transcription factor NXF antibody
- bHLHe79 antibody
- Class E basic helix-loop-helix protein 79 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Npas4 (green, C) using ab242003 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 1 hr at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
Anti-Npas4 antibody [S408-79] (ab242003) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 6 minutes
Primary incubation for 16 hours at 4°C.
Secondary incubatin for 1 hr at RT.
Blocked for 1 hr at RT.
ab242003 has not yet been referenced specifically in any publications.