Anti-NPAT antibody [2648C6a] (ab70595)
Key features and details
- Mouse monoclonal [2648C6a] to NPAT
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-NPAT antibody [2648C6a]
See all NPAT primary antibodies -
Description
Mouse monoclonal [2648C6a] to NPAT -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment (Human) from an internal sequence of NPAT.
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Positive control
- Recombinant human NPAT.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
2648C6a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 240 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 240 kDa. |
Target
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Relevance
NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry. -
Cellular localization
Nuclear -
Alternative names
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
Images
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Anti-NPAT antibody [2648C6a] (ab70595) + immunising recombinant fragment
Predicted band size: 240 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (4)
ab70595 has been referenced in 4 publications.
- Quinodoz SA et al. RNA promotes the formation of spatial compartments in the nucleus. Cell 184:5775-5790.e30 (2021). PubMed: 34739832
- Qian W et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). PubMed: 29532868
- Huang X & Lyu J Tumor suppressor function of miR-483-3p on breast cancer via targeting of the cyclin E1 gene. Exp Ther Med 16:2615-2620 (2018). PubMed: 30186493
- Mano M et al. Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction. Proc Natl Acad Sci U S A 112:11276-81 (2015). PubMed: 26305933