Overview

  • Product name

    Anti-NPAT antibody [2648C6a]
    See all NPAT primary antibodies
  • Description

    Mouse monoclonal [2648C6a] to NPAT
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human) from an internal sequence of NPAT.

  • Positive control

    • Recombinant human NPAT.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    2648C6a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Relevance

    NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • E14 antibody
    • nuclear protein ataxia telangiectasia locus antibody
    • nuclear protein in the AT region antibody

Images

  • Anti-NPAT antibody [2648C6a] (ab70595) + immunising recombinant fragment

    Predicted band size: 240 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

This product has been referenced in:

  • Qian W  et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). Read more (PubMed: 29532868) »
  • Huang X & Lyu J Tumor suppressor function of miR-483-3p on breast cancer via targeting of the cyclin E1 gene. Exp Ther Med 16:2615-2620 (2018). Read more (PubMed: 30186493) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70595.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up