Key features and details
- Mouse monoclonal [2648C6a] to NPAT
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NPAT antibody [2648C6a]
See all NPAT primary antibodies
DescriptionMouse monoclonal [2648C6a] to NPAT
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment (Human) from an internal sequence of NPAT.
- Recombinant human NPAT.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab70595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.|
|Dot blot||Use at an assay dependent dilution.|
RelevanceNPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
Anti-NPAT antibody [2648C6a] (ab70595) + immunising recombinant fragment
Predicted band size: 240 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab70595 has been referenced in 3 publications.
- Qian W et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). PubMed: 29532868
- Huang X & Lyu J Tumor suppressor function of miR-483-3p on breast cancer via targeting of the cyclin E1 gene. Exp Ther Med 16:2615-2620 (2018). PubMed: 30186493
- Mano M et al. Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction. Proc Natl Acad Sci U S A 112:11276-81 (2015). PubMed: 26305933