Anti-NPAT antibody (ab99436)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)
  • Immunoprecipitation - Anti-NPAT antibody (ab99436)

Key features and details

  • Rabbit polyclonal to NPAT
  • Suitable for: IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NPAT antibody
    See all NPAT primary antibodies

  • Description

    Rabbit polyclonal to NPAT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 700-750 of Human NPAT (NP_002510.2).

  • Positive control

    • Human 293T cell line.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab99436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • E14 antibody
      • nuclear protein ataxia telangiectasia locus antibody
      • nuclear protein in the AT region antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-NPAT antibody (ab99436)
      Immunoprecipitation - Anti-NPAT antibody (ab99436)
      Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

      Western Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml

      developed using the ECL technique

      Exposure time : 30 seconds

      Predicted band size : 154 kDa

    References (0)

    Publishing research using ab99436? Please let us know so that we can cite the reference in this datasheet.

    ab99436 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab99436.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com