Overview

  • Product name
    Anti-NPAT antibody
    See all NPAT primary antibodies
  • Description
    Rabbit polyclonal to NPAT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 700-750 of Human NPAT (NP_002510.2).

  • Positive control
    • Human 293T cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab99436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance
      NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.
    • Cellular localization
      Nuclear
    • Database links
      • Alternative names
        • E14 antibody
        • nuclear protein ataxia telangiectasia locus antibody
        • nuclear protein in the AT region antibody

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB.
      • Immunoprecipitation - Anti-NPAT antibody (ab99436)
        Immunoprecipitation - Anti-NPAT antibody (ab99436)
        Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

        Western Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml

        developed using the ECL technique

        Exposure time : 30 seconds

        Predicted band size : 154 kDa

      References

      ab99436 has not yet been referenced specifically in any publications.

      Publishing research using ab99436? Please let us know so that we can cite the reference in this datasheet.

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