Anti-NPAT antibody (ab99436)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NPAT antibody
See all NPAT primary antibodies -
Description
Rabbit polyclonal to NPAT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 700-750 of Human NPAT (NP_002510.2).
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Positive control
- Human 293T cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab99436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-5 µg/mg of lysate. |
Application notes
Is unsuitable for WB.
Target
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Relevance
NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4863 Human
- Omim: 601448 Human
- SwissProt: Q13632 Human
- SwissProt: Q14207 Human
- SwissProt: Q16580 Human
- Unigene: 171061 Human
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Alternative names
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (ab99436)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-NPAT antibody (ab99436)Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in laneWestern Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 154 kDa
Datasheets and documents
References
ab99436 has not yet been referenced specifically in any publications.
