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    npff1-receptor-antibody-ab45350.pdf

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Anti-NPFF1 Receptor antibody (ab45350)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Western blot - Anti-NPFF1 Receptor antibody (ab45350)
  • Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)

Key features and details

  • Rabbit polyclonal to NPFF1 Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NPFF1 Receptor antibody
    See all NPFF1 Receptor primary antibodies
  • Description

    Rabbit polyclonal to NPFF1 Receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human NPFF1 Receptor aa 350 to the C-terminus.
    (Peptide available as ab45349)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.

      This antibody gave a positive signal in the following cell lines: PC12.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
WB
Human
Application Abreviews Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q9GZQ6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64106 Human
    • Entrez Gene: 64107 Rat
    • Omim: 607448 Human
    • SwissProt: Q9GZQ6 Human
    • SwissProt: Q9EP86 Rat
    • Unigene: 302026 Human
    • Unigene: 64483 Rat
    • Alternative names

      • G Protein Coupled Receptor 147 antibody
      • G-protein coupled receptor 147 antibody
      • GPR147 antibody
      • Neuropeptide FF receptor 1 antibody
      • NPFF1 antibody
      • NPFF1_HUMAN antibody
      • NPFF1R1 antibody
      • NPFFR1 antibody
      • RFamide related peptide receptor OT7T022 antibody
      • RFamide-related peptide receptor OT7T022 antibody
      see all

    Images

    • Western blot - Anti-NPFF1 Receptor antibody (ab45350)
      Western blot - Anti-NPFF1 Receptor antibody (ab45350)

      The peptide blocking data above shows that ab45350 detects a protein of approximately 64 kDa, thought to be the NPFF1 Receptor.  It is possible that this band is higher than expected due to modifications at potential glycosylation sites.

    • Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)
      Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)

      ICC/IF image of ab45350 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab45350? Please let us know so that we can cite the reference in this datasheet.

    ab45350 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Question

    Phoned to inquire about a 100% Abreview code for ab45350

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2012

    Answer

    DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab45350 inICC/IF on mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF on mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 13 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-NPFF1 Receptor antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Oct 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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