- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-NPFF1 Receptor antibody
See all NPFF1 Receptor primary antibodies -
DescriptionRabbit polyclonal to NPFF1 Receptor
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide derived from within residues 350 to the C-terminus of Human NPFF1 Receptor.
(Peptide available as ab45349.)
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.
This antibody gave a positive signal in the following cell lines: PC12.
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab45350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionReceptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 64106 Human
- Entrez Gene: 64107 Rat
- Omim: 607448 Human
- SwissProt: Q9GZQ6 Human
- SwissProt: Q9EP86 Rat
- Unigene: 302026 Human
- Unigene: 64483 Rat
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Alternative names
- G Protein Coupled Receptor 147 antibody
- G-protein coupled receptor 147 antibody
- GPR147 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)ICC/IF image of ab45350 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab45350 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"