Overview

  • Product name

    Anti-NPFF2 Receptor antibody
    See all NPFF2 Receptor primary antibodies
  • Description

    Rabbit polyclonal to NPFF2 Receptor
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes human NPFF2 (45 kDa).
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    N-terminal sequence MNEKWDTNSSENWHPI and C-terminal sequence ELVMEELKETTNSSEI of human NPFF2.

Properties

Applications

Our Abpromise guarantee covers the use of ab1402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Western Blot: use at 1:500-1:1,000 dilution.
    ELISA: use at 1:1,000-1:5,000 dilution
  • Target

    • Function

      Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    • Tissue specificity

      Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • G protein coupled receptor 74 antibody
      • G protein coupled receptor HLWAR 77 antibody
      • G protein coupled receptor HLWAR77 antibody
      • G-protein coupled receptor 74 antibody
      • G-protein coupled receptor HLWAR77 antibody
      • GPR 74 antibody
      • GPR74 antibody
      • HLWAR 77 antibody
      • HLWAR77 antibody
      • Neuropeptide FF 2 antibody
      • Neuropeptide FF receptor 2 antibody
      • Neuropeptide G protein coupled receptor antibody
      • Neuropeptide G-protein coupled receptor antibody
      • NPFF 2 antibody
      • NPFF R2 antibody
      • NPFF2 antibody
      • NPFF2_HUMAN antibody
      • NPFFR 2 antibody
      • Npffr2 antibody
      • NPGPR antibody
      see all

    References

    ab1402 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. I was able to obtain the following information regarding these two antibodies which will hopefully be of assistance to you. For ab1402, the Western blots were done with human brain lysate; the antibody was used at a 1:500 dilution. Phase extraction was not used, and a band of approximately 45 kD was detected. For ab3899 - Human brain tissue lysate (I am not sure what area of brain tissue was used) was homogenized in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 1 mM PMSF, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). After centrifugation, protein concentration was determined and the tissue lysate was stored in 1 x SDS sample buffer (60 mM Tris-HCl pH 6.8, 10% glycerol, 2% SDS, 0.03% bromophenol blue with 0.3% b-mercaptoethanol. If you have any further questions, please let me know.

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