Overview

  • Product name

    Anti-NPHP1 antibody
  • Description

    Rabbit polyclonal to NPHP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NPHP1 aa 1-109.
    Database link: O15259

  • Positive control

    • IHC-P: Human tonsil and colon tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab211422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.

Target

  • Function

    Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis.
  • Tissue specificity

    Widespread expression, with highest levels in pituitary gland, spinal cord, thyroid gland, testis, skeletal muscle, lymph node and trachea. Weakly expressed in heart, kidney and pancreas. Expressed in nasal epithelial cells (at protein level).
  • Involvement in disease

    Nephronophthisis 1
    Senior-Loken syndrome 1
    Joubert syndrome 4
  • Sequence similarities

    Belongs to the nephrocystin-1 family.
    Contains 1 SH3 domain.
  • Developmental stage

    During in vitro ciliogenesis translocalizes from the cytoplasm to the ciliary transition zone during epithelial cell polarization.
  • Domain

    The SH3 domain mediates the stable interaction with Cas.
  • Post-translational
    modifications

    Phosphorylation by CK2 is required for the interaction with PACS1 and the targeting to the base region of cilia.
  • Cellular localization

    Cell junction, adherens junction. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme. Cell junction, tight junction. Colocalizes with E-cadherin and BCAR1 at or near the cell-cell adherens junctions (By similarity). Localized to respiratory cilia axoneme. Localized to the transition zone of respiratory cilia, photoreceptor-connecting cilia and renal monocilia. In cultured renal cells, it localizes diffusely in the cytoplasm but, as cells approach confluence, it accumulates to basolateral tight junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • JBTS4 antibody
    • Juvenile nephronophthisis 1 protein antibody
    • Nephrocystin 1 antibody
    • Nephrocystin-1 antibody
    • nephronophthisis 1 (juvenile) antibody
    • Nephronophthisis antibody
    • NPH1 antibody
    • NPHP1 antibody
    • NPHP1_HUMAN antibody
    • SLSN1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling NPHP1 with ab211422 at 10 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling NPHP1 with ab211422 at 10 µg/ml.

References

ab211422 has not yet been referenced specifically in any publications.

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