Key features and details
- Rabbit polyclonal to NPHP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NPHP1 antibody
DescriptionRabbit polyclonal to NPHP1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NPHP1 aa 1-109.
Database link: O15259
- IHC-P: Human tonsil and colon tissues.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityCaprylic Acid - Ammonium Sulfate precipitation
Our Abpromise guarantee covers the use of ab211422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.|
FunctionTogether with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis.
Tissue specificityWidespread expression, with highest levels in pituitary gland, spinal cord, thyroid gland, testis, skeletal muscle, lymph node and trachea. Weakly expressed in heart, kidney and pancreas. Expressed in nasal epithelial cells (at protein level).
Involvement in diseaseNephronophthisis 1
Senior-Loken syndrome 1
Joubert syndrome 4
Sequence similaritiesBelongs to the nephrocystin-1 family.
Contains 1 SH3 domain.
Developmental stageDuring in vitro ciliogenesis translocalizes from the cytoplasm to the ciliary transition zone during epithelial cell polarization.
DomainThe SH3 domain mediates the stable interaction with Cas.
modificationsPhosphorylation by CK2 is required for the interaction with PACS1 and the targeting to the base region of cilia.
Cellular localizationCell junction, adherens junction. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme. Cell junction, tight junction. Colocalizes with E-cadherin and BCAR1 at or near the cell-cell adherens junctions (By similarity). Localized to respiratory cilia axoneme. Localized to the transition zone of respiratory cilia, photoreceptor-connecting cilia and renal monocilia. In cultured renal cells, it localizes diffusely in the cytoplasm but, as cells approach confluence, it accumulates to basolateral tight junctions.
- Information by UniProt
- JBTS4 antibody
- Juvenile nephronophthisis 1 protein antibody
- Nephrocystin 1 antibody
ab211422 has not yet been referenced specifically in any publications.