Overview

  • Product name
  • Description
    Mouse polyclonal to NPHS2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-315 of Human NPHS2 (AAH29141.1).

  • Positive control
    • Human placenta tissue lysate; NPHS2 transfected 293T cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab168625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.

Target

  • Function
    Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • Tissue specificity
    Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • Involvement in disease
    Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Images

  • Anti-NPHS2 antibody (ab168625) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 34 kDa

  • All lanes : Anti-NPHS2 antibody (ab168625) at 1 µg/ml

    Lane 1 : NPHS2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 34 kDa

References

This product has been referenced in:
  • Haley KE Podocyte injury elicits loss and recovery of cellular forces. Sci Adv. 4:eaap8030 (2018). ICC/IF ; Human . Read more (PubMed: ) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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