Overview

  • Product name
  • Description
    Rabbit polyclonal to NPHS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NPHS2 aa 367-383 conjugated to keyhole limpet haemocyanin (Cysteine residue). Synthetic peptide conjugated to KLH by an N-terminal Cysteine residue linker.
    Sequence:

    SKPVEPLNPKKKDSPML

  • Positive control
    • Whole extract of rat glomeruli and human or rat kidney frozen sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab50339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • Tissue specificity
    Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • Involvement in disease
    Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

     

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

  • ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Wang P  et al. Knockdown of NUP160 inhibits cell proliferation, induces apoptosis, autophagy and cell migration, and alters the expression and localization of podocyte associated molecules in mouse podocytes. Gene 664:12-21 (2018). WB, IF . Read more (PubMed: 29704630) »
  • Liu B  et al. Zhen-wu-tang protects against podocyte injury in rats with IgA nephropathy via PPAR?/NF-?B pathway. Biomed Pharmacother 101:635-647 (2018). WB . Read more (PubMed: 29518610) »
See all 32 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Ms. Jeremy Song

Verified customer

Submitted May 16 2012

Answer

Thank you for your reply.
I have issued you the testing discount codes for ab82108 and ab82107 for testing in rat and pig. Please see below for the information.
For ab50339 I am sending you a testing discount code for testing in IHC-P. Since IHC-P is so far untested you can submit an Abreview for IHC-P with rat. If you like you can also try it on pig tissue, and then submit a separate Abreview for this species as well. For each published Abreview will receive Abpoints that can be used on a future order.
If you have not yet done so, you can submit for ab85379 each an Abreview for IHC-P with rat and also for IHC-Fr with rat.
Here are the testing discount codes:
DISCOUNT CODE: xxx for ab50339 for IHC-P
DISCOUNT CODE: xxx for ab82108 for rat, pig
DISCOUNT CODE: xxx for ab82107 for rat, pig
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab82108 and ab82107 in rat and pig, and for ab50339 fo rtetsing in IHC-P. Each code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat/pig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.
Please let me know if there is anything else I can do for you.

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Answer

Thank you for your email.
I am sorry to hear that these antibodies are not working as expected.
As for ab58968, we had replaced this antibody free of charge with ab85379. Since ab85379 was not tested in rat, sheep, and pig tissue, I had sent you a testing discount code. The code (xxx) is still valid until its expiration date of xxx.
Which species did you try ab85379 on?
As for ab50339, it has only been tested in WB, IHC-Fr, ICC/IF, but not paraffin embedded sections. It might work on paraffin sections, but we have no data to confirm this as it was not yet tested.
I'd be happy, though, to give you a testing discount code for trying it in IHC-P. Would you use it on the same species as ab85379?
I look forward to hear back and assist you further.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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