Overview

  • Product name
  • Description
    Rabbit polyclonal to NPHS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NPHS2 aa 367-383 conjugated to keyhole limpet haemocyanin (Cysteine residue). Synthetic peptide conjugated to KLH by an N-terminal Cysteine residue linker.
    Sequence:

    SKPVEPLNPKKKDSPML

  • Positive control
    • Whole extract of rat glomeruli and human or rat kidney frozen sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab50339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
  • Tissue specificity
    Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
  • Involvement in disease
    Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder.
  • Sequence similarities
    Belongs to the band 7/mec-2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
    • nephrosis 2, idiopathic, steroid resistant antibody
    • NPHS2 antibody
    • NPHS2 gene antibody
    • PDCN antibody
    • PODO_HUMAN antibody
    • Podocin antibody
    • SRN1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

     

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 µg/ml.  

  • ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Wang P  et al. Knockdown of NUP160 inhibits cell proliferation, induces apoptosis, autophagy and cell migration, and alters the expression and localization of podocyte associated molecules in mouse podocytes. Gene 664:12-21 (2018). WB, IF . Read more (PubMed: 29704630) »
  • Liu B  et al. Zhen-wu-tang protects against podocyte injury in rats with IgA nephropathy via PPAR?/NF-?B pathway. Biomed Pharmacother 101:635-647 (2018). WB . Read more (PubMed: 29518610) »
See all 32 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Kidney)
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Ms. Jeremy Song

Verified customer

Submitted May 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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