Overview

  • Product name

    Anti-NPL antibody
  • Description

    Rabbit polyclonal to NPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human NPL aa 17-77.
    Database link: Q9BXD5

  • Positive control

    • WB: Mouse brain lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface.
  • Tissue specificity

    Isoform 2 is expressed in placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte.
  • Pathway

    Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 1/5.
  • Sequence similarities

    Belongs to the DHDPS family. NanA subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610033B02Rik antibody
    • C112 antibody
    • C1orf13 antibody
    • Dihydrodipicolinate synthase antibody
    • Dihydrodipicolinate synthetase homolog 1 antibody
    • MGC149582 antibody
    • MGC61869 antibody
    • N-acetylneuraminate lyase antibody
    • N-acetylneuraminate pyruvate-lyase antibody
    • N-acetylneuraminic acid aldolase antibody
    • NAL antibody
    • NALase antibody
    • NPL antibody
    • NPL_HUMAN antibody
    • NPL1 antibody
    • RP11-249O6.1 antibody
    • Sialate lyase antibody
    • Sialate-pyruvate lyase antibody
    • Sialic acid aldolase antibody
    • Sialic acid lyase antibody
    see all

Images

  • Anti-NPL antibody (ab223521) at 1/2000 dilution + Mouse brain lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NPL (green) using ab223521 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

References

ab223521 has not yet been referenced specifically in any publications.

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