The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
Protein degradation; proteasomal ubiquitin-dependent pathway.
Belongs to the NPL4 family. Contains 1 RanBP2-type zinc finger.
Binds ubiquitinated proteins via its RanBP2-type zinc finger.
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
Detection of NPL4 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 µg/mg lysate for IP. Lane 2 represents control IgG IP. An anti-NPL4 antibody was used at 0.1 µg/ml for subsequent western blot detection.
Detection: Chemiluminescence with exposure time of 3 minutes.