Overview

  • Product name
  • Description
    Rabbit polyclonal to NPL4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human NPL4 aa 525-575. The exact sequence is proprietary.
    Database link: NP_060391.2

  • Positive control
    • 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab176383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
    • Tissue specificity
      Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
    • Pathway
      Protein degradation; proteasomal ubiquitin-dependent pathway.
    • Sequence similarities
      Belongs to the NPL4 family.
      Contains 1 RanBP2-type zinc finger.
    • Domain
      Binds ubiquitinated proteins via its RanBP2-type zinc finger.
    • Cellular localization
      Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1499 antibody
      • NPL4 antibody
      • NPL4, S. cerevisiae, homolog of antibody
      • NPL4_HUMAN antibody
      • nploc4 antibody
      • nuclear protein localization 4 homolog (S. cerevisiae) antibody
      • Nuclear protein localization 4 homolog antibody
      • Nuclear protein localization protein 4 homolog antibody
      • Nuclear protein localization protein 4, S. cerevisiae, homolog of antibody
      • Protein NPL4 antibody
      see all

    Images

    • Detection of NPL4 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 µg/mg lysate for IP. Lane 2 represents control IgG IP. An anti-NPL4 antibody was used at 0.1 µg/ml for subsequent western blot detection.

      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab176383 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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