Anti-NPP-7 antibody (ab231811)
Key features and details
- Rabbit polyclonal to NPP-7
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-NPP-7 antibody
See all NPP-7 primary antibodies -
Description
Rabbit polyclonal to NPP-7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Rat NPP-7 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q5EZ72 -
Positive control
- WB: Recombinant rat NPP-7 and rat liver tissue lysate. IHC-P: Rat intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231811 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. -
Tissue specificity
Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. -
Post-translational
modificationsN-glycosylated; required for activity and transport to the plasma membrane. -
Cellular localization
Membrane. Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 303729 Rat
- SwissProt: Q5EZ72 Rat
- Unigene: 211790 Rat
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Alternative names
- ALK SMase antibody
- Alk-SMase antibody
- Alkaline sphingomyelin phosphodiesterase antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat intestine tissue stained for NPP-7 using ab231811 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-NPP-7 antibody (ab231811) at 2 µg/ml + Recombinant rat NPP-7
Predicted band size: 50 kDa -
Anti-NPP-7 antibody (ab231811) at 2 µg/ml + Rat liver lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution
Predicted band size: 50 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231811 has not yet been referenced specifically in any publications.