Key features and details
- Rabbit polyclonal to NPP-7
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-NPP-7 antibody
See all NPP-7 primary antibodies
DescriptionRabbit polyclonal to NPP-7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Recombinant full length protein (His-T7-tag) corresponding to Rat NPP-7 aa 22-439. (Expressed in E.coli).
GPVQRQQQHKLLLVSFDGFRWNYDQDVETPNLDSMAQEGVKARYMTPAFV TMTSPCHFTLVTGKYIENHGVVHNMFYNTTNKVRLPYHATLGIQRWWDNG SIPIWITAQRQGLKTGSFFYPGGNVTYQGEAVTMSRKEGVLHNYKNETEW RANVDTVMKWFTEEDVSLVTLYFGEPDSTGHKYGPESQERKDMVKQVDRT VGYLRDSIKRHHLTDSLNLIITSDHGMTTVNKKASDLVEFHKFPNFTFRD IEFELLDYGPNGMLIPKEGMLEKVYSVLKDAHPRLHVYKKEDFPKTFHYA NNPRITSLLMYSDLGYVIHGRVNVQFNSGEHGFDNQDMDMKTIFRAVGPS FKAGLEVEPFESVHVYELMCQLLGIVPEPNDGHPGVLQPMLRSGSPLSRQ HHLVVVLMGILTGLAKVV
Database link: Q5EZ72
- WB: Recombinant rat NPP-7 and rat liver tissue lysate. IHC-P: Rat intestine tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231811 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionConverts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Tissue specificityDetected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
Sequence similaritiesBelongs to the nucleotide pyrophosphatase/phosphodiesterase family.
modificationsN-glycosylated; required for activity and transport to the plasma membrane.
Cellular localizationMembrane. Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex.
- Information by UniProt
- ALK SMase antibody
- Alk-SMase antibody
- Alkaline sphingomyelin phosphodiesterase antibody
Formalin-fixed, paraffin-embedded rat intestine tissue stained for NPP-7 using ab231811 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-NPP-7 antibody (ab231811) at 2 µg/ml + Recombinant rat NPP-7
Predicted band size: 50 kDa
Anti-NPP-7 antibody (ab231811) at 2 µg/ml + Rat liver lysate
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/1000 dilution
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231811 has not yet been referenced specifically in any publications.