Overview

  • Product name

    Anti-NPP-7 antibody [EPR12329]
    See all NPP-7 primary antibodies
  • Description

    Rabbit monoclonal [EPR12329] to NPP-7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NPP-7 aa 350-450 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6UWV6

  • Positive control

    • Caco 2, HT-29 and Human fetal liver lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
    • Tissue specificity

      Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
    • Sequence similarities

      Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
    • Post-translational
      modifications

      N-glycosylated; required for activity and transport to the plasma membrane.
    • Cellular localization

      Membrane. Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALK SMase antibody
      • Alk-SMase antibody
      • Alkaline sphingomyelin phosphodiesterase antibody
      • E-NPP 7 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase 7 antibody
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 antibody
      • ENPP7 antibody
      • ENPP7_HUMAN antibody
      • Intestinal alkaline sphingomyelinase antibody
      • NPP-7 antibody
      see all

    Images

    • All lanes : Anti-NPP-7 antibody [EPR12329] (ab174281) at 1/10000 dilution

      Lane 1 : Caco 2 lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa

    References

    ab174281 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174281.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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