RecombinantRabMAb

Recombinant Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)

Submit a review Q&A (6)References (2)
Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
  • Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
  • Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
  • Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NPR-A + NPR-B antibody [EPR5719(2)]

  • Description

    Rabbit monoclonal [EPR5719(2)] to NPR-A + NPR-B

  • Host species

    Rabbit

  • Specificity

    The immunogen for this product shares 95% homology with NPR-A

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NPR-B aa 800-900. The exact sequence is proprietary.
    (Peptide available as ab150296)

  • Positive control

    • Human artery, fetal kidney, fetal brain, Mouse brain, Mouse heart, Rat brain, and Rat heart tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab139188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 117 kDa.Can be blocked with NPR-B peptide (ab150296).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Human artery lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 117 kDa

    • Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 117 kDa

    • Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      All lanes : Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

      Lane 1 : GST-tagged recombinant human Natriuretic Peptide Receptor A protein (aa 495 to 1061)
      Lane 2 : GST-tagged recombinant human Natriuretic Peptide Receptor B protein (aa 479 to 1047)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 117 kDa
      Observed band size: 88 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking buffer and concentration: 5% NFDM/TBST

      Loading Control: Anti-GST antibody, ab111947

    • Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)
      Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188)

    References (2)

    Publishing research using ab139188? Please let us know so that we can cite the reference in this datasheet.

    ab139188 has been referenced in 2 publications.

    • Wang Q  et al. Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice. PeerJ 7:e6942 (2019). PubMed: 31143551
    • Ding DZ  et al. C-type natriuretic peptide prevents angiotensin II-induced atrial connexin 40 and 43 dysregulation by activating AMP-activated kinase signaling. Mol Med Rep 20:5091-5099 (2019). PubMed: 31638216

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question



    Inquiry: Does the antibody target the extracellular or intracellular domain of the protein?

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    Answer

    Thank you for your inquiry. This antibody targets an intracellular portion of the protein. If you have any other questions or concerns please feel free to contact us.

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    Question

    for ab139188: IHC-P is listed as unsuitable
    what samples and species was the Ab tested on in IHC-P?

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    Answer


    The lab let me know that when they tested ab139188 in IHC-P, they used a wide variety of normal and cancer tissue arrays (usually human only). They did not see any staining.

    Read More

    Question

    We are interested in you anti-NPRB antibodies (ab139188). We are wondering which secondary antibody works better for ab139188? Thanks a lot.

    Read More
    Answer

    Thank you for contacting us. Any anti-rabbit antibody should work well with this product. Generally I recommend using a pre-absorbed antibody such as ab7078. Pre-adsorbed secondary antibodies are less likely to interact with endogenous

    Read More

    Question

    send Uniprot entry
    blocking peptides available?
    for ab79164: will test in mouse

    Read More
    Answer

    Thank you for your patience.

    I have heard back from the lab that the blocking peptide for ab116680 has been added to the catalog as ab151530: https://www.abcam.com/natriuretic-peptide-receptor-a-ab151530.html

    The blocking peptide for ab139188 has been added as ab150296: https://www.abcam.com/NPR-B-peptide-ab150296.html

    Both items will be in stock in 1-2 weeks once an order is placed.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    send Uniprot entry
    blocking peptides available?
    for ab79164: will test in mouse

    Read More
    Answer

    Thank you for your patience.

    The immunogen peptides for ab116680 and ab139188 would be available, and I have requested that they will be added to the catalog. I will let you know when I have more information regarding catalog number, prize etc.

    I hope this information is already of some help to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    WB, mouse
    send Uniprot entry
    blocking peptides available?
    for ab79164: will test in mouse

    Read More
    Answer

    Thank you for your phone call.

    The UniProt database entry for Natriuretic Peptide Receptor A is as follows: http://www.uniprot.org/uniprot/P18293

    For ab79164, the immunogen peptide is in the catalog as product ab95057.
    For the other 2 antibodies I have inquired with the lab and am waiting to hear back from them. I will let you know when I do.

    The discount code for trying ab79164 in mouse is as follows:
    DISCOUNT CODE: xxx
    Expiration date: xxx
    Value: xxx

    I am very pleased to hear you would like to accept our offer and test ab79164 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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