Anti-NPR-C antibody (ab97389)
Key features and details
- Rabbit polyclonal to NPR-C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NPR-C antibody
See all NPR-C primary antibodies -
Description
Rabbit polyclonal to NPR-C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human NPR-C aa 265-514.
Database link: NP_000899 -
Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lines IHC-P: U87 xenograft ICC/IF: Hela cell
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General notes
This product was previously labelled as Natriuretic Peptide Receptor C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. -
Sequence similarities
Belongs to the ANF receptor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281358 Cow
- Entrez Gene: 4883 Human
- Entrez Gene: 18162 Mouse
- Entrez Gene: 25339 Rat
- Omim: 108962 Human
- SwissProt: P10730 Cow
- SwissProt: P17342 Human
- SwissProt: P70180 Mouse
see all -
Alternative names
- ANP C antibody
- ANP-C antibody
- ANPC antibody
see all
Images
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Western blot - Anti-NPR-C antibody (ab97389)Anti-NPR-C antibody (ab97389) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
The lower image (Panel 2) was merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.
Protocols
References (2)
ab97389 has been referenced in 2 publications.
- Zayed MA et al. Natriuretic Peptide Receptor-C is Up-Regulated in the Intima of Advanced Carotid Artery Atherosclerosis. J Med Surg Pathol 1:N/A (2016). IHC-P ; Human . PubMed: 27547837
- Peake N et al. Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Res Ther 15:R76 (2013). WB, IF . PubMed: 23883591
