Anti-NPR-C antibody (ab97389)

Reviews (2)Q&A (1)References (2)

Overview

  • Product name

    Anti-NPR-C antibody
    See all NPR-C primary antibodies

  • Description

    Rabbit polyclonal to NPR-C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human NPR-C aa 265-514.
    Database link: NP_000899

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lines IHC-P: U87 xenograft ICC/IF: Hela cell

  • General notes

     This product was previously labelled as Natriuretic Peptide Receptor C

     

Properties

Applications

Our Abpromise guarantee covers the use of ab97389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.

  • Sequence similarities

    Belongs to the ANF receptor family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

Images

  • Western blot - Anti-NPR-C antibody (ab97389)
    Western blot - Anti-NPR-C antibody (ab97389)
    Anti-NPR-C antibody (ab97389) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)
    Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
    The lower image (Panel 2) was merged with DNA probe.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.

References

This product has been referenced in:

  • Zayed MA  et al. Natriuretic Peptide Receptor-C is Up-Regulated in the Intima of Advanced Carotid Artery Atherosclerosis. J Med Surg Pathol 1:N/A (2016). IHC-P ; Human . Read more (PubMed: 27547837) »
  • Peake N  et al. Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Res Ther 15:R76 (2013). WB, IF . Read more (PubMed: 23883591) »
See all 2 Publications for this product

Publishing research using ab97389? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic Peptide Receptor C antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
Permeabilization
No
Specification
Placenta
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 02 2016

Western blot abreview for Anti-Natriuretic Peptide Receptor C antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Intestinal Myofibroblasts)
Loading amount
30 µg
Specification
Intestinal Myofibroblasts
Gel Running Conditions
Reduced Denaturing (7.5 % gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Dec 18 2012

Question

Would like a testing code to try this antibody with mouse samples.

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Answer

DISCOUNT CODE: ABTRIAL-TBSWD
Expiration date: March 1, 2013
Value: $337

I am very pleased to hear you would like to test ab97389 in with mouse samples. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab97389 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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