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    npr-c-antibody-ab97389.pdf

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Anti-NPR-C antibody (ab97389)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (2)

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Abpromise

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Western blot - Anti-NPR-C antibody (ab97389)
  • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)

Key features and details

  • Rabbit polyclonal to NPR-C
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human NPR-C protein (ab114355)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NPR-C antibody
    See all NPR-C primary antibodies
  • Description

    Rabbit polyclonal to NPR-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human NPR-C aa 265-514.
    Database link: NP_000899

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lines IHC-P: U87 xenograft ICC/IF: Hela cell
  • General notes

     This product was previously labelled as Natriuretic Peptide Receptor C

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Blood
    • Blood Pressure regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human NPR-C protein (ab114355)

Applications

Our Abpromise guarantee covers the use of ab97389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
  • Sequence similarities

    Belongs to the ANF receptor family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P17342 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281358 Cow
    • Entrez Gene: 4883 Human
    • Entrez Gene: 18162 Mouse
    • Entrez Gene: 25339 Rat
    • Omim: 108962 Human
    • SwissProt: P10730 Cow
    • SwissProt: P17342 Human
    • SwissProt: P70180 Mouse
    • SwissProt: P41740 Rat
    • Unigene: 237028 Human
    • Unigene: 619466 Human
    • Unigene: 25259 Mouse
    • Unigene: 11297 Rat
    see all
  • Alternative names

    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

Images

  • Western blot - Anti-NPR-C antibody (ab97389)
    Western blot - Anti-NPR-C antibody (ab97389)
    Anti-NPR-C antibody (ab97389) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)
    Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
    The lower image (Panel 2) was merged with DNA probe.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPR-C antibody (ab97389)

    ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab97389? Please let us know so that we can cite the reference in this datasheet.

    ab97389 has been referenced in 2 publications.

    • Zayed MA  et al. Natriuretic Peptide Receptor-C is Up-Regulated in the Intima of Advanced Carotid Artery Atherosclerosis. J Med Surg Pathol 1:N/A (2016). IHC-P ; Human . PubMed: 27547837
    • Peake N  et al. Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Res Ther 15:R76 (2013). WB, IF . PubMed: 23883591

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Natriuretic Peptide Receptor C antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
    Permeabilization
    No
    Specification
    Placenta
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 02 2016

    Western blot abreview for Anti-Natriuretic Peptide Receptor C antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Intestinal Myofibroblasts)
    Loading amount
    30 µg
    Specification
    Intestinal Myofibroblasts
    Gel Running Conditions
    Reduced Denaturing (7.5 % gel)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 18 2012

    Question

    Would like a testing code to try this antibody with mouse samples.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 29 2012

    Answer

    DISCOUNT CODE: ABTRIAL-TBSWD
    Expiration date: March 1, 2013
    Value: $337

    I am very pleased to hear you would like to test ab97389 in with mouse samples. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab97389 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Oct 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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