Recombinant
RabMAb

Recombinant Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)

Overview

  • Product name

    Anti-NPR-C antibody [EPR12716] - C-terminal
    See all NPR-C primary antibodies
  • Description

    Rabbit monoclonal [EPR12716] to NPR-C - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human NPR-C aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P17342

  • Positive control

    • Human fetal kidney, Human placenta, and HeLa lysates; HeLa cells.
  • General notes

     This product was previously labelled as Natriuretic Peptide Receptor C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
    • Sequence similarities

      Belongs to the ANF receptor family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANP C antibody
      • ANP-C antibody
      • ANPC antibody
      • ANPR-C antibody
      • ANPRC antibody
      • ANPRC_HUMAN antibody
      • Atrial natriuretic peptide C type receptor antibody
      • Atrial natriuretic peptide clearance receptor antibody
      • Atrial natriuretic peptide receptor 3 antibody
      • Atrial natriuretic peptide receptor type C antibody
      • Atrionatriuretic peptide receptor C antibody
      • C5orf23 antibody
      • FLJ14054 antibody
      • GUCY2B antibody
      • MGC22189 antibody
      • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
      • Natriuretic peptide receptor C/guanylate cyclase C antibody
      • NPR 3 antibody
      • NPR C antibody
      • NPR-C antibody
      • Npr3 antibody
      • NPRC antibody
      see all

    Images

    • All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)

      All lanes : Human tissue lysate

      Lysates/proteins at 1/10 dilution per lane.

      Secondary
      All lanes : HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 60 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 60 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 60 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

    • All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human placenta lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa

    • Flow Cytometric analysis of permeabilized HeLa cells labeling NPR-C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab177954 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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