Product nameAnti-NPSR1 antibody
See all NPSR1 primary antibodies
DescriptionRabbit polyclonal to NPSR1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rhesus monkey
Synthetic peptide corresponding to Human NPSR1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Heart Tissue Lysate. IHC-P (FFPE): Human Colon (Normal)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab92425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
RelevanceNeuropeptide S receptor 1 (GPR154) is a receptor for Neuropeptide S. It is expressed on the epithelia of several organs including the intestine, and appears to be upregulated in inflammation. It is upregulated in macrophages after antigen challenge and is involved in the pathogenesis of asthma and other IgE-mediated diseases.
Cellular localizationCell Membrane and Cytoplasmic
- ASRT2 antibody
- G protein coupled receptor 154 antibody
- G protein coupled receptor for asthma susceptibility antibody
Anti-NPSR1 antibody (ab92425) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ICC/IF image of ab92425 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92425, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of NPSR1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92425, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Slattery DA et al. Selective breeding for high anxiety introduces a synonymous SNP that increases neuropeptide S receptor activity. J Neurosci 35:4599-613 (2015). Read more (PubMed: 25788677) »
- Zhang S et al. Persistent nociception induces anxiety-like behavior in rodents: role of endogenous neuropeptide S. Pain 155:1504-15 (2014). Read more (PubMed: 24793908) »