• Product name

  • Description

    Rabbit polyclonal to NPSR1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human NPSR1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101418)

  • Positive control

    • This antibody gave a positive signal in Human Heart Tissue Lysate. IHC-P (FFPE): Human Colon (Normal)



Our Abpromise guarantee covers the use of ab92425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance

    Neuropeptide S receptor 1 (GPR154) is a receptor for Neuropeptide S. It is expressed on the epithelia of several organs including the intestine, and appears to be upregulated in inflammation. It is upregulated in macrophages after antigen challenge and is involved in the pathogenesis of asthma and other IgE-mediated diseases.
  • Cellular localization

    Cell Membrane and Cytoplasmic
  • Database links

  • Alternative names

    • ASRT2 antibody
    • G protein coupled receptor 154 antibody
    • G protein coupled receptor for asthma susceptibility antibody
    • G protein coupled receptor PGR14 antibody
    • GPR 154 antibody
    • gpr154 antibody
    • GPRA antibody
    • MVTR antibody
    • Neuropeptide S receptor 1 antibody
    • Neuropeptide S receptor antibody
    • nps receptor antibody
    • NPSR 1 antibody
    • NPSR antibody
    • NPSR1 antibody
    • PGR 14 antibody
    • PGR14 antibody
    • Vasopressin receptor related receptor 1 antibody
    • VRR 1 antibody
    • VRR1 antibody
    see all


  • Anti-NPSR1 antibody (ab92425) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 10 minutes
  • ICC/IF image of ab92425 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92425, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of NPSR1 staining in Human Colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92425, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


This product has been referenced in:

  • Slattery DA  et al. Selective breeding for high anxiety introduces a synonymous SNP that increases neuropeptide S receptor activity. J Neurosci 35:4599-613 (2015). Read more (PubMed: 25788677) »
  • Zhang S  et al. Persistent nociception induces anxiety-like behavior in rodents: role of endogenous neuropeptide S. Pain 155:1504-15 (2014). Read more (PubMed: 24793908) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain)
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 06 2016

Western blot
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% polyacrylamide Bio-Rad ReadyGel, Tris buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Michael Saul

Verified customer

Submitted Aug 11 2011

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