Overview

  • Product name

  • Description

    Rabbit polyclonal to NPTX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide near the center of the human NPTX2

Properties

Applications

Our Abpromise guarantee covers the use of ab69858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • ab69858 at 5µg/ml, staining NPTX2 by IHC-P.
  • Western blot analysis of NPTX2 expression in mouse brain tissue lysate with ab69858 at 1µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • Lane 1 : Anti-NPTX2 antibody (ab69858) at 0.5 µg
    Lane 2 : Anti-NPTX2 antibody (ab69858) at 1 µg/ml

    All lanes : Mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 47 kDa

  • Immunofluorescence of NPTX2 in Human Brain cells using ab69858 at 20 ug/ml.

References

This product has been referenced in:

  • Xu C  et al. NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/ß-catenin pathway via FZD6. Cell Death Dis 10:217 (2019). Read more (PubMed: 30833544) »
  • Tang CZ  et al. Overexpression of microRNA-301b accelerates hippocampal microglia activation and cognitive impairment in mice with depressive-like behavior through the NF-?B signaling pathway. Cell Death Dis 10:316 (2019). Read more (PubMed: 30962417) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

The immunogen sequence for ab69858 is proprietary information but it is within amino acids 181-240 of NP_002514. This is the alignment with NPTX1: [ :*:*- .* * *::*]. The asterisks represent amino acids that are identical in the two proteins.

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