Overview

  • Product name

    Anti-NPTX2 antibody [EPR15618]
    See all NPTX2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15618] to NPTX2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NPTX2 aa 50-250. The exact sequence is proprietary.
    Database link: P47972

  • Positive control

    • Human pancreas, testis and fetal brain lysates; mouse and rat brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 47-55 kDa (predicted molecular weight: 47 kDa).

Target

Images

  • All lanes : Anti-NPTX2 antibody [EPR15618] (ab191563) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human pancreas lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47-55 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-NPTX2 antibody [EPR15618] (ab191563) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa
    Observed band size: 47-55 kDa why is the actual band size different from the predicted?

References

ab191563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Robert Fenster

Verified customer

Submitted Aug 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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